Chan, Sunney I.

Biological Chemistry

Chan, Sunney I. (陳長謙)

    Chan, Sunney I. 's Lab
B.S. (1957) and Ph.D. (1960), University of California, Berkeley.;National Science Foundation Postdoctoral Fellow, 1960-1961, Harvard University.;Assistant Professor, 1961-1963, University of California, Riverside.;Assistant Professor, 1963-1964; Associate Professor, 1964-68; Professor, 1968-1992; George Grant Hoag Professor of Biophysical Chemistry, 1992-2001; Hoag Professor, Emeritus, 2002-, California Institute of Technology.;Honorary Professor, 1996-2015, Chinese University of Hong Kong. ;Chair Distinguished Professor of Chemistry, 2007-2010; Distinguished Research Chair Professor, 2010-2016, National Taiwan University.;Honorary Chair Professor, 2012-14, National Ping Tung University of Education.;Chair Professor of Bioinorganic Chemistry, 2013-16, National Chung Hsing University. ;Distinguished Research Fellow and Director, 1997-1999; Distinguished Research Fellow, 1999-2011; Distinguished Visiting Fellow, 2011-15, Institute of Chemistry, Academia Sinica.;Vice President, 1999-2003, Academia Sinica.;Director, National Research Program of Genomic Medicine, 2001-03, National Science Council, Republic of China.;
Research Interests

Physical biochemistry; structure and function of membrane proteins; magnetic resonance spectroscopy; X-ray spectroscopy; bioinorganic chemistry and biocatalysis; rational design of catalysts for Green Chemistry; protein folding; bioenergetics.

A tricopper complex capable of efficient conversion of methane into methanol at room temperature

A tricopper complex capable of efficient conversion of methane into methanol at room temperature

Selected Publications
  1. Fellow, American Physical Society,1987.
  2. Elected Member of Academia Sinica, 1988.
  3. ASCIT Teaching Award, California Institute of Technology, 1991.
  4. Fellow of the American Association for the Advancement of Science, 1992.
  5. Wilson T. S. Wang Distinguished International Professor, Chinese University of Hong Kong, 1993.
  6. Richard M. Badger Teaching Prize, California Institute of Technology, 1994.
  7. Doctor of Science honoris causea, Hong Kong Baptist University, 2003.
  8. William C. Rose Award, American Society of Biochemistry and Molecular Biology, 2004.
  9. Member, Academy of Sciences for the Developing World, 2004.
  10. Fellow, Biophysical Society USA, 2004.
  11. CBNet Award in Biophysics, Biophysical Society, 2005.
  12. Fellow of the American Academy of Arts and Sciences, 2010.
  13. Fellow of the International Academy of Physical Sciences, 2011.

Selected Publications
  1. Nagababu, P.; Yu, S. S.-F.; Maji, S.; Ramu, R.; Chan, S. I. An Efficient Catalyst for Controlled Oxidation of C1-C6 Hydrocarbons under Ambient Conditions. Catal. Sci. Tech. 2014, DOI: 10.1039/c3cy00884c.
  2. Chen, P. P.-Y.; Nagababu, P.; Yu, S. S.-F.; Chan, S. I. Development of the Tricopper Cluster as a Catalyst for Efficient Conversion of Methane into Methanol. ChemCatChem minireview 2014, 6, 429. DOI: 10.1002/cctc.201300473.
  3. Chan, S.I. Opportunities for Innovation in Chemical Education. The Chemical Education Journal 2013, 15, 1.
  4. Pham, M. D.; Yu, S. S.-F.; Han, C.-C.; Chan, S. I. Improved Mass Spectrometric Analysis of Membrane Proteins Based on Rapid and Versatile Sample Preparation on Nanodiamond Particles. Anal. Chem. 2013, 85, 6748.
  5. Chan, S. I.; Lu, Y.-J.; Nagababu, P.; Maji, S.; Hung, M.-C.; Lee, M. M.; Hsu, I.-J.; Pham, M. D.; Lai, J. C.-H.; Ng, K. Y.; Ramalingam, S.; Yu, S. S.-F.; Chan, M. K. Efficient Oxidation of Methane to Methanol by Dioxygen Mediated by Tricopper Clusters. Angew. Chem. Int. Ed. 2013, 52, 3731.
  6. Nagababu, P.; Maji, S.; Kumar, M. P.; Chen, P. P.-Y.; Yu, S. S.-F.; Chan, S. I. Efficent Room-temperature Oxidation of Hydrocarbons Mediated by Tricopper Cluster Complexes with Different Ligands. Adv. Synth. Catal. 2012, 354, 3275.
  7. Chan, S. I.; Chien, C. Y.-C.; Yu, C. S.-C.; Nagababu, P.; Maji, S.; Chen, P. P.-Y. Efficient Catalytic Oxidation of Hydrocarbons Mediated by a Tricopper Cluster under Mild Conditions. J. Catalysis 2012, 293, 186.
  8. Maji, S.; Lee, J. C.-M.; Lu, Y.-J.; Chen, C.-L.; Hung, M.-C.; Chen, P. P.-Y.; Yu, S. S.-F.; Chan, S. I. Activation of a Trinuclear CuICuICuI Cluster Capable of Mediating Facile Oxidation of Organic Substrates: Competition between O-Atom Transfer and Abortive Inter-complex Reduction. Chem. Eur. J. 2012, 18, 3955.
  9. Huang, J. J.-H.; Larsen, R. W.; Chan, S. I. The Interplay of Turn Formation and Hydrophobic Interactions on the Early Kinetic Events in Protein Folding. Chem. Comm. (Feature Article), (Royal Society of Chemistry) 2012, 48, 487.
  10. Chan, S. I. Proton Pumping in Cytochrome c Oxidase: The Coupling between Proton and Electron Gating. Proc. Nat. Acad. Sci. USA 2010, 107, 8505.
  11. Chan, S. I. A Physical Chemist's Expedition to Explore the World of Membrane Proteins. Annual Review of Biophysics 2009, 38, 1.

Update: 2015-03-23

Journal Paper
  1. Liu IPC, Chen PPY, Chan SI Models for the trinuclear copper(II) cluster in the particulate methane monooxygenase from methanotrophic bacteria: Synthesis, spectroscopic and theoretical characterization of trinuclear copper(II) complexes. Comptes Rendus Chimie 2012-0315, 214-224.
  2. Lo FC, Lee JF, Liaw WF, Hsu IJ, Tsai YF, Chan SI, Yu SS The metal core structures in the recombinant Escherichia coli transcriptional factor SoxR. CHEMISTRY-A EUROPEAN JOURNAL 2012-01, 18(9)2665-2577.
  3. Chen KH, Wu HH, Ke SF, Rao YT, Tu CM, Chen YP, Kuei KH, Chen YS, Wang VC, Kao WC, Chan SI Bacteriohemerythrin bolsters the activity of the particulate methane monooxygenase (pMMO) in Methylococcus capsulatus (Bath).. Journal of inorganic biochemistry 2012111, 10-7.
  4. Lin KF, Sun CS, Huang YC, Chan SI, Koubek J, Wu TH, Huang JJ Cotranslational protein folding within the ribosome tunnel influences trigger-factor recruitment.. Biophysical journal 2012102(12), 2818-27.
  5. Maji S, Lee JC, Lu YJ, Chen CL, Hung MC, Chen PP, Yu SS, Chan SI Dioxygen activation of a trinuclear Cu(I)Cu(I)Cu(I) cluster capable of mediating facile oxidation of organic substrates: competition between O-atom transfer and abortive intercomplex reduction.. Chemistry (Weinheim an der Bergstrasse, Germany) 201218(13), 3955-68.
  6. Huang JJ, Larsen RW, Chan SI The interplay of turn formation and hydrophobic interactions on the early kinetic events in protein folding.. Chemical communications 201248(4), 487-497.
  7. Jou-Yin Fang, Yuan-Lan Chiang, Yin-Cheng Hsieh, Vincent C.-C. Wang, Yen-Chieh Huang, Phimonphan Chuankhayan, Ming-Chi Yang, Ming-Yih Liu, Sunney I. Chan, and Chun-Jung Chen Crystallization of Adenylylsulfate Reductase from Desulfovibrio gigas: A Strategy Based on Controlled Protein Oligomerization. CRYSTAL GROWTH & DESIGN 2011-0411, 2127-2134.
  8. Pei-Fen Su, Chin-Jin Li, Chih-Chien Hsu, Spencer Benson, Sheng-Yang Wang, Kandan Aravindaram, Sunney I. Chan, Shih-Hsiung Wu, Feng-Ling Yang, Wen-Ching Huang, Lie-Fen Shyur, and Ning-Sun Yang Dioscorea Phytocompounds Enhance Murine Splenocyte Proliferation Ex Vivo and Improve Regeneration of Bone Marrow Cells In Vivo. EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE 2011, 1-11.
  9. Chan SI, Nguyen HH, Chen KH, Yu SS Overexpression and purification of the particulate methane monooxygenase from Methylococcus capsulatus (Bath).. Methods in enzymology 2011495, 177-193.
  10. Wu LL, Yang CL, Lo FC, Chiang CH, Chang CW, Ng KY, Chou HH, Hung HY, Chan SI, Yu SS Tuning the regio- and stereoselectivity of C-H activation in n-octanes by cytochrome P450 BM-3 with fluorine substituents: evidence for interactions between a C-F bond and aromatic pi systems.. Chemistry - A European Journal 201117(17), 4774-4787.
  11. Chan SI Proton pumping in cytochrome c oxidase: the coupling between proton and electron gating.. Proceedings of the National Academy of Sciences of the United States of America 2010107(19), 8505-8506.
  12. Chan SI A physical chemist's expedition to explore the world of membrane proteins.. Annual review of biophysics 200938, 1-27.
  13. Chen HL, Huang YF, Lim TS, Su CH, Chen PH, Su AC, Wong KT, Chao TC, Chan SI, Fann W Excited-state backbone twisting of polyfluorene as detected from photothermal after-effects.. JOURNAL OF PHYSICAL CHEMISTRY B 2009113(25), 8527-31.
  14. Lee MM, Isaza CE, White JD, Chen RP, Liang GF, He HT, Chan SI, Chan MK Insight into the substrate length restriction of M32 carboxypeptidases: characterization of two distinct subfamilies.. Proteins 200977(3), 647-657.
  15. Ho CC, Lee LY, Huang KT, Lin CC, Ku MY, Yang CC, Chan SI, Hsu RL, Chen RP Tuning the conformational properties of the prion peptide.. Proteins 200976(1), 213-225.
  16. Isaza CE, Zhong X, Rosas LE, White JD, Chen RP, Liang GF, Chan SI, Satoskar AR, Chan MK A proposed role for Leishmania major carboxypeptidase in peptide catabolism.. Biochemical and biophysical research communications 2008373(1), 25-29.
  17. Chan SI, Yu SS Controlled oxidation of hydrocarbons by the membrane-bound methane monooxygenase: the case for a tricopper cluster.. Accounts of chemical research 200841(8), 969-979.
  18. Kao WC, Wang VC, Huang YC, Yu SS, Chang TC, Chan SI Isolation, purification and characterization of hemerythrin from Methylococcus capsulatus (Bath).. Journal of inorganic biochemistry 2008102(8), 1607-1614.
  19. Ng KY, Tu LC, Wang YS, Chan SI, Yu SS Probing the hydrophobic pocket of the active site in the particulate methane monooxygenase (pMMO) from Methylococcus capsulatus (Bath) by variable stereoselective alkane hydroxylation and olefin epoxidation.. Chembiochem : a European journal of chemical biology 20089(7), 1116-1123.
  20. Chen PP, Yang RB, Lee JC, Chan SI Facile O-atom insertion into C-C and C-H bonds by a trinuclear copper complex designed to harness a singlet oxene.. Proceedings of the National Academy of Sciences of the United States of America 2007104(37), 14570-14575.
  21. Chen SH, Liao HK, Chang CY, Juo CG, Chen JH, Chan SI, Chen YJ Targeted protein quantitation and profiling using PVDF affinity probe and MALDI-TOF MS.. Proteomics 20077(17), 3038-3050.
  22. Yu SS, Ji CZ, Wu YP, Lee TL, Lai CH, Lin SC, Yang ZL, Wang VC, Chen KH, Chan SI The C-terminal aqueous-exposed domain of the 45 kDa subunit of the particulate methane monooxygenase in Methylococcus capsulatus (Bath) is a Cu(I) sponge.. Biochemistry 200746(48), 13762-74.
  23. Tseng, CF, Burger, A, Mislin, GLA, Schalk, IJ, Yu, SSF, Chan, SI and Abdallah, MA Bacterial siderophores: the solution stoichiometry and coordination of the Fe(III) complexes of pyochelin and related compounds. JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY 200611(4), 419-432.
  24. Chen, PPY and Chan, SI Theoretical modeling of the hydroxylation of methane as mediated by the particulate methane monooxygenase. JOURNAL OF INORGANIC BIOCHEMISTRY 2006100(4), 801-809.
  25. Liang, FC, Chen, RPY, Lin, CC, Huang, KT and Chan, SI Tuning the conformation properties of a peptide by glycosylation and phosphorylation. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 2006342(2), 482-488.
  26. Y.Y. Chen, S.Y. Luo, S.C. Hung, S.I. Chan* and D.L. Tzou 13C solid-state NMR chemical shift anisotropy analysis of the anomeric carbon in carbohydrates.. Carbohydr Res. 2005340(4), 723-729.
  27. N.N.-W. Kuo, J. Huang, J. Miksovska, P.-Y. Chen, R. Larsen and S.I. Chan* Effects of turn stability on the kinetics of refolding of a hairpin in β-sheet.. J. Am. Chem. Soc. 2005127(48), 16945-16954.
  28. S.I. Chan*, J.J.-T. Huang, R.W. Larsen, R.S. Rock and K.C. Hansen “Early Kinetics Events in Protein Folding: The Development and Applications of Caged Peptides” in Dynamic Studies in Biology. Phototriggers, Photoswitches, and Caged Biomolecules. Goeldner, M. and Givens. R. (eds), Wiley-VCH GmbH & Co. (Germany).  2005, 479-494.
  29. Chen RP, Huang JJ, Chen HL, Jan H, Velusamy M, Lee CT, Fann W, Larsen RW, Chan SI Measuring the refolding of beta-sheets with different turn sequences on a nanosecond time scale.. Proceedings of the National Academy of Sciences of the United States of America 2004101(19), 7305-10.
  30. M.S. Vinchurkar, K.H.C. Chen, S.S.F. Yu, S.J. Kuo, H.C. Chiu, S.H. Chien and S.I. Chan* Polarized ATR-FTIR spectroscopy of the membrane-embedded domains of the particulate methane monooxygenase.. Biochemistry 200443, 13283-13292.
  31. C.L. Chen, K.H.C. Chen, S.C. Ke, S.S.F. Yu and S.I. Chan* Preparation and characterization of a (Cu, Zn)-pMMO from Methylococcus capsulatus (Bath).. J. Inorg. Biochem. 200498, 2125-2130.
  32. W.C. Kao, Y.R. Chen, E.C. Yi, H. Lee, Q. Tian, K.M. Wu, S.F. Tsai, S.S.F. Yu, Y.J. Chen, R. Aebersold and S.I. Chan* Quantitative proteomic analysis of metabolic regulation by copper ions in Methylococcus capulatus (Bath).. J. Biol. Chem. 2004279, 51554-51560.
  33. R.S. Rock, K.C. Hansen, R.W. Larsen and S.I. Chan* Rapid photochemical triggering of protein unfolding in a nondenaturing environment.. J. Chem. Phys. 2004307, 201-208.
  34. Pavlova SV, Chen KH, Chan SI Spectroscopic characterization of the oxo-transfer reaction from a bis(mu-oxo)dicopper(III) complex to triphenylphosphine.. Dalton transactions (Cambridge, England : 2003) 2004(20), 3261-72.
  35. S.I. Chan*, K.H.C. Chen, S.S.F. Yu, C.L. Chen and S.S.J. Kuo Toward delineating the structure and function of the particulate methane monooxygenase from methanotrophic bacteria.. Biochemistry 200443, 4421-4430.
  36. S.S.F. Yu, L.Y. Wu, K.H.C. Chen, M.Y.H. Tseng, Y.S. Wang, C.F. Tseng, Y.J. Chen, D.S. Huang and S.I. Chan* Production of high quality pMMO in high yields from Methylococcus capsulatus (Bath) with a hollow-fiber membrane bioreactor.. J. Bacteriol. 2003185, 5915-5924.
  37. S.S.F. Yu, L.Y. Wu, K.H.C. Chen, W.I. Luo, D.S. Huang and S.I. Chan* The stereospecific hydroxylation of [2,2-2h2]butane and chiral dideuteriobutanes by the particulate methane monooxygenase from Methylococcus capsulatus (Bath).. J. Biol. Chem. 2003278, 40658-40669.