Lee, Hsien-Ming

Chemical Biology and Bioorganic Chemistry

Lee, Hsien-Ming (李賢明)

    886-2-27898620
    886-2-27831237
    leehm
Assistant Research Fellow (2011-);B.S., National Chao Tung University (1991 - 1995);Ph.D., Purdue University (1998 – 2004);Postdoctoral Fellow, Albert Einstein College of Medicine (2004 - 2007);Postdoctoral Fellow, University of North Carolina at Chapel Hill (2007 – 2010) ;
Research Interests

Using chemistry as a tool to observe, to probe and even to alter the biochemical reactions in the living organism is a very exciting topic in the area of chemical biology. We are very interested in any type of vesicle design that can deliver drugs or functional macromolecules into the living cells, such as surface functionalized liposomes, nanoparticles and cell penetrating peptides. Our lab also focuses on the design and the synthesis of many types of biosensor that can report to us what is going on in the living cells. These two directions will allow us, in the foreseeable future, to “run” or “test” our biochemical reactions in the living organisms.

  • Development of IR biomedical imaging and IR photo-releasing applications based on upconversion nanocrystals / nanoparticles.
  • Development of fluorescence-reporting biosensors.
  • Protein chemical modifications for novel protein functions.
  • Cellular drug delivery using peptides, liposomes, and nanoparticles.
  • Development of cell-penetrating / organelle-targeting peptides as cellular delivery vesicles.



Selected Publications
  1. Gao, H.-D. ; Chiang, C.-W.; Thanasekaran, P.; Hong, J.-L.; Liu, Y.-C.; Chang, Y.-H.; Lee, H.-M. Construction of a Near-Infrared-Activatable Enzyme Platform to Remotely Trigger Intracellular Signal Transduction Using an Upconversion Nanoparticle. ACS NANO 2015, 9, 7041. >
  2. He, R.-Y.; Huang, Y.-C.; Chiang, C.-W.; Tsai, Y.-J.; Ye, T.-J.; Gao, H.-D.; Wu, C.-Y.; Lee, H.-M.; Huang, J. J.-T. Characterization and real-time imaging of the FTLD-related protein aggregation induced by amyloidogenic peptides. Chem. Comm. 2015, 51, 8652.
  3. Hsieh, C.-W.; Chu, C.-H.; Lee, H.-M.; Yang, W.-Y. Triggering Mitophagy with Far-Red Light.  Sci. Rep. 2015, 5, 10376.
  4. Liu, G. C.-H.; Chen, B. P.-W.; Ye, N. T.-J.; Wang, C.-H.; Chen, W.; Lee, H.-M.; Chan, S. I., Huang, J. J.-T. Delineating the membrane-disrupting and seeding properties of the TDP-43 amyloidogenic core. Chem. Commun. 2013, 49, 11212.
  5. Priestman, M. A.; Shell, T. A.; Sun, L.; Lee, H.-M.; Lawrence, D. S. Merging of Confocal and Caging Technologies: Selective Three-Color Communication with Profluorescent Reporters. Angew. Chem. Int. Ed. 2012, 51, 1.
  6. Lee, H.-M.; Xu, W.; Lawrence, D. S. Construction of a Photoactivatable Profluorescent Enzyme Via Propinquity Labeling. J. Am. Chem. Soc. 2011, 133, 2331.
  7. Lee, H.-M.; Priestman, M. A.; Lawrence, D. S. Light-Mediated Spatial Control via Photolabile Fluorescently Quenched Peptide Cassettes. J. Am. Chem. Soc. 2010, 132, 1446.
  8. Wakata, A.; Lee, H.-M.; Toutchkine, A.; Schmidt, M.; Lawrence, D. S. Simultaneous Fluorescent Monitoring of Proteasomal Subunit Catalysis. J. Am. Chem. Soc. 2010, 132, 1578.
  9. Lee, H.-M.; Larson, D. R.; Lawrence, D. S. Illuminating the Chemistry of Life: Design, Synthesis, and Applications of “Caged” and Related Photoresponsive Compounds. ACS Chem. Biol. 2009, 4, 409.
    (Ranked # 3 of the “Most Read Articles in ACS Chemical Biology” in 2009)
  10. Lee, H.-M.; Chmielewski, J. A. Liposomal Cargo Unloading Induced by pH-Sensitive Peptides. J. Peptide Res. 2005, 65, 355-363
  11. Satish Nayak,; Lee, H.-M.; Chmielewski, J. A.; Lyon, A. Folate-Mediated Cell Targeting and Cytotoxicity Using Thermoresponsive Microgels. J. Am. Chem. Soc. 2004, 126, 10258.
  12. Lee, H.-M.; Kim, Y. W.; Baird, J. K. Electrophoretic Mobility and Zeta-potential of Lysozyme Crystals in Aqueous Solutions of Some 1 : 1 Electrolytes. J. Cryst. Growth 2001, 232, 294.

Update: 2016-01-06

Journal Paper
  1. Hua-De Gao, Pounraj Thanasekaran, Chao-Wei Chiang, Jia-Lin Hong, Yen-Chun Liu, Yu-Hsu Chang, and Hsien-Ming Lee * Construction of a Near-Infrared-Activatable Enzyme Platform to Remotely Trigger Intracellular Signal Transduction Using an Upconversion Nanoparticle. ACS Nano 2015-06 9(7), 7041–7051.
  2. Cheng-Wei Hsieh, Chih-Hang Chu, Hsien-Ming Lee, and Wei-Yuan Yang Triggering Mitophagy with Far-Red Light. Scientific Reports 2015-055, 10376.
  3. He, R.-Y.; Huang, Y.-C.; Chiang, C.-W.; Tsai, Y.-J.; Ye, T.-J.; Gao, H.-D.; Wu, C.-Y.; Lee, H.-M.*; Huang, J. J.-T.* Characterization and real-time imaging of the FTLD-related protein aggregation induced by amyloidogenic peptides. CHEMICAL COMMUNICATIONS 2015-0451, 8652-8655.
  4. Gerard Chun-Hao Liu , Bryan Po-Wen Chen , Nancy Ting-Juan , Chih-Hsien Wang , Wenlung Chen , Hsien-Ming Lee , Sunney I. Chan and Joseph Jen-Tse Huang  Delineating the membrane-disrupting and seeding properties of the TDP-43 amyloidogenic core. CHEMICAL COMMUNICATIONS 2013-1049, 11212-11214.
  5. Priestman MA, Shell TA, Sun L, Lee HM, Lawrence DS Merging of confocal and caging technologies: selective three-color communication with profluorescent reporters.. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 201251(31), 7684-7.