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李賢明 (Hsien-Ming Lee)

化學生物和生物有機化學

李賢明 (Hsien-Ming Lee)

    886-2-55728620
    886-2-27831237
    leehm*
助研究員(100年迄今);國立交通大學學士 (80 – 84年);美國普度大學博士 (87 – 93年);美國愛因斯坦醫學院生化系博士後研究員 (93 – 96年);美國北卡萊納州教堂山分校藥學系及化學系合聘博士後研究員(96 – 99年);台灣神隆製藥 (99-100年) ;
研究領域

在化學生物的領域中, 利用化學為工具來了解, 改變細胞內生化反應, 甚至到巨觀上用來控制細胞行為, 是個非常有趣的課題. 所以設計並合成具有生物活性的大分子,是我們實驗室主要的工作.

我們的核心技術

(化學端)
1. 微脂體(liposome)及膠束(micelle)為奈米醫學藥物載具
2. 多功能的多肽設計
3. 多功能的化學修飾改造蛋白質
4. 生物相容波段螢光(近紅外線及上轉換波段)
(儀器端)
5. 細胞精細顯微注射
6. 高通量細胞生物影像
7. 螢光半衰期生物影像
8. 多肽快速合成
9. 各類高階顯微鏡

有了這些化學及儀器工具在手, 我們要解決的問題

1. 精準奈米醫學載具--如何設計出更聰明的奈米藥物載具,使其作用在患部卻不會影響健康組織
2. 診斷類生物影像--發展可以專一在患部造成明顯核磁訊號對比顯影(MRI contrast)
3. 篩檢類生物影像--發展可以幫助藥物開發人員篩檢藥物療效的細胞組織層面螢光影像
4. 可受光遙控的生物分子--在活體內進行空間時間受控制的生化反應

只要您是對生物有興趣的化學人或對化學有興趣的生物人,具有完整大學部化學,材料或生命科學相關科系專業訓練,有清楚的邏輯及科學思考能力,我們都竭誠歡迎加入我們的研究行列。只要您是上面所描述的人,我們實驗室永遠會用政府允許的最高薪資級別聘請。不論是助理或是研究生,隨時可應徵。請直接跟李賢明博士聯絡。



代表作
    JOURNAL ARTICLES:
  1. Gao, H.-D.; Thanasekaran, P.; Chen, T.-H.; Chang, Y.-H.; Chen, Y.-J.; Lee, H.-M. An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation.  JoVE. 2017 (Accepted)
  2. Zhang, S.; Chen, T.-H.; Lee, H.-M.; Bi, J.; Ghosh, A.; Fang, M.; Qian , Z.; Xie, F.; Ainsely, J.; Christov, C.; Luo, F.-T.; Zhao, F.; Liu, H. Luminescent Probes for Sensitive Detection of pH Changes in Live Cells through Two Near-infrared Luminescence Channels.  ACS Sensors2017 (Accepted)
  3. He, R.-Yu; Chao, S.-H.; Tsai, Y.-J.; Lee, C.-C.; Yu, C.-Y.; Gao, H.-D.; Huang, Y.-A.; Hwang, E.; Lee, H.-M.; Huang, J. J.-T. Photo-Controllable Probe Spatiotemporally Induces Neurotoxic Fibrillar Aggregates and Impairs Nucleocytoplasmic Trafficking.  ACS NANO, 201(Accepted)
  4. Yadav, K.; Chou, A.-C.; Ulaganathan, R. K.; Gao, H.-D.; Lee, H.-M.; Pan, C.-Y.; Chen, Y.-T. Targeted and Efficient Activation of Channelrhodopsins Expressed in Living Cells Via Specifically-Bound Upconversion Nanoparticles. Nanoscale2017 (Accepted)
  5. Gao, H.-D. ; Chiang, C.-W.; Thanasekaran, P.; Hong, J.-L.; Liu, Y.-C.; Chang, Y.-H.; Lee, H.-M. Construction of a Near-Infrared-Activatable Enzyme Platform to Remotely Trigger Intracellular Signal Transduction Using an Upconversion Nanoparticle. ACS NANO 2015, 9, 7041.
  6. He, R.-Y.; Huang, Y.-C.; Chiang, C.-W.; Tsai, Y.-J.; Ye, T.-J.; Gao, H.-D.; Wu, C.-Y.; Lee, H.-M.; Huang, J. J.-T. Characterization and real-time imaging of the FTLD-related protein aggregation induced by amyloidogenic peptides. Chem. Comm. 2015, 51, 8652.
  7. Hsieh, C.-W.; Chu, C.-H.; Lee, H.-M.; Yang, W.-Y. Triggering Mitophagy with Far-Red Light.  Sci. Rep. 2015, 5, 10376.
  8. Liu, G. C.-H.; Chen, B. P.-W.; Ye, N. T.-J.; Wang, C.-H.; Chen, W.; Lee, H.-M.; Chan, S. I., Huang, J. J.-T. Delineating the membrane-disrupting and seeding properties of the TDP-43 amyloidogenic core. Chem. Commun. 2013, 49, 11212.
  9. Priestman, M. A.; Shell, T. A.; Sun, L.; Lee, H.-M.; Lawrence, D. S. Merging of Confocal and Caging Technologies: Selective Three-Color Communication with Profluorescent Reporters. Angew. Chem. Int. Ed. 2012, 51, 1.
  10. Lee, H.-M.; Xu, W.; Lawrence, D. S. Construction of a Photoactivatable Profluorescent Enzyme Via Propinquity Labeling. J. Am. Chem. Soc. 2011, 133, 2331.
  11. Lee, H.-M.; Priestman, M. A.; Lawrence, D. S. Light-Mediated Spatial Control via Photolabile Fluorescently Quenched Peptide Cassettes. J. Am. Chem. Soc. 2010, 132, 1446.
  12. Wakata, A.; Lee, H.-M.; Toutchkine, A.; Schmidt, M.; Lawrence, D. S. Simultaneous Fluorescent Monitoring of Proteasomal Subunit Catalysis. J. Am. Chem. Soc. 2010, 132, 1578.
  13. Lee, H.-M.; Larson, D. R.; Lawrence, D. S. Illuminating the Chemistry of Life: Design, Synthesis, and Applications of “Caged” and Related Photoresponsive Compounds. ACS Chem. Biol. 2009, 4, 409.
    (Ranked # 3 of the “Most Read Articles in ACS Chemical Biology” in 2009)
  14. Lee, H.-M.; Chmielewski, J. A. Liposomal Cargo Unloading Induced by pH-Sensitive Peptides. J. Peptide Res. 2005, 65, 355-363
  15. Satish Nayak,; Lee, H.-M.; Chmielewski, J. A.; Lyon, A. Folate-Mediated Cell Targeting and Cytotoxicity Using Thermoresponsive Microgels. J. Am. Chem. Soc. 2004, 126, 10258.
  16. Lee, H.-M.; Kim, Y. W.; Baird, J. K. Electrophoretic Mobility and Zeta-potential of Lysozyme Crystals in Aqueous Solutions of Some 1 : 1 Electrolytes. J. Cryst. Growth 2001, 232, 294.
    BOOK CHAPTER:
  1. Thanasekaran, P.; Gao, H.-D.; Lee, H.-M. Phosphors, Upconversion Nano Particles, Quantum Dots and Their Applications: Upconversion Nanoparticle as a Platform for Photoactivation; Liu, R.-S. (eds), Chapter 34, Springer Publication, China, 2015.

Update: 2017-07-18

期刊論文
  1. Kanchan Yadav, Ai-Chuan Chou, Rajesh Kumar Ulaganathan, Hua-De Gao, Hsien-Ming Lee*, Chien-Yuan Pan*, Yit-Tsong Chen* Targeted and efficient activation of channelrhodopsins expressed in living cells via specifically-bound upconversion nanoparticles. Nanoscale 20179(27), 9457-9466.
  2. Hua-De Gao, Pounraj Thanasekaran, Chao-Wei Chiang, Jia-Lin Hong, Yen-Chun Liu, Yu-Hsu Chang, and Hsien-Ming Lee * Construction of a Near-Infrared-Activatable Enzyme Platform to Remotely Trigger Intracellular Signal Transduction Using an Upconversion Nanoparticle. ACS Nano 2015-06 9(7), 7041–7051.
  3. Cheng-Wei Hsieh, Chih-Hang Chu, Hsien-Ming Lee, and Wei-Yuan Yang Triggering Mitophagy with Far-Red Light. Scientific Reports 2015-055, 10376.
  4. He, R.-Y.; Huang, Y.-C.; Chiang, C.-W.; Tsai, Y.-J.; Ye, T.-J.; Gao, H.-D.; Wu, C.-Y.; Lee, H.-M.*; Huang, J. J.-T.* Characterization and real-time imaging of the FTLD-related protein aggregation induced by amyloidogenic peptides. CHEMICAL COMMUNICATIONS 2015-0451, 8652-8655.
  5. Gerard Chun-Hao Liu , Bryan Po-Wen Chen , Nancy Ting-Juan , Chih-Hsien Wang , Wenlung Chen , Hsien-Ming Lee , Sunney I. Chan and Joseph Jen-Tse Huang  Delineating the membrane-disrupting and seeding properties of the TDP-43 amyloidogenic core. CHEMICAL COMMUNICATIONS 2013-1049, 11212-11214.
  6. Priestman MA, Shell TA, Sun L, Lee HM, Lawrence DS Merging of confocal and caging technologies: selective three-color communication with profluorescent reporters.. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 201251(31), 7684-7.
專書(論文集)之一章
  1. Thanasekaran, P.; Gao, H.-D.; Lee, H.-M.*, 2016, “Upconversion Nanoparticle as a Platform for Photoactivation isbn”, editor(s): Liu, R.-S., Phosphors, Upconversion Nano Particles, Quantum Dots and Their Applications (Volume 2), pp. 391, SINGAPORE: SPRINGER.