Yu, Steve Sheng-Fa

生物有機化學及生物無機化學

俞聖法 (Yu, Steve Sheng-Fa)

    886-2-27898650
    886-2-27831237
    sfyu
副研究員(100年迄今);助研究員(92-100年);清華大學化學學士(76至80年)及化學博士(81至87年);中央研究院化學研究所博士後研究(88至92年);成功大學化學系合聘助理教授(94年迄今);清華大學材料科學工程學系兼任助理教授(94至100年);
研究領域

發展含氟烷類化合物為金屬蛋白催化烷烴活化的研究探針
烷烴化合物的碳氫鍵活化一直是有機化學家十分重要的挑戰課題,以傳統的化學轉化方法,則必需在高溫高壓下的環境方可達成,然而,自然界則存在可活化甲烷甚至是辛烷的細菌,它們均具備相對應的酵素來轉化這些高能的分子。
我們實驗室以嗜甲烷菌系中的甲烷單加氧酵素、假單胞菌系中的末端氧化酵素與巨大芽孢菌系中的細胞色素P-450 BM-3為研究材料,利用所設計的含氟探針,一方面將研究所獲取的酵素動力學與光學活性選擇性等資料,配合分子模擬的方法找出其選擇性與結構的相關性,並探索在疏水性基質活化中心周圍,控制位置選擇性與立體選擇性的要素。另一方面,則以點突變、隨機突變或部分表現的方法,來營造酵素本體抑或是活性中心附近變異;其目的,可以使得酵素對於基質上的選擇有更佳的專一性,而產物的選擇可以有更多的多樣性。由於更多變異性的產生,可以藉此從事更多相關生物物理與生物化學的探討,讓我們對其活化碳氫鍵的機轉有更深入的瞭解,這樣的研究將有助於相關新型態催化劑的研發,可導向新穎的有機化學合成模式,並將有機會對環境污染物的降解有所助益,符合目前永續綠色化學的研究目標。
其中,我們置備了各式的雙氟取代的辛烷化合物,這些含氟的烷類探針可以選擇性地被末端氧化酵素活化,專一性地從事遠端活化生成相對應的一級醇,這類的羥基化反應可以利用全細胞催化的策略來完成。

調整具備烷烴活化能力的金屬蛋白從事天然物或精緻化學品其位置選擇性或立體選擇性之合成轉換
經基因轉殖後之巨大芽孢菌系之細胞色素P450 BM-3突變株能夠位置選擇性地將氟化辛烷轉化成為相對應的二級醇,尤其是在四號位取代的雙氟辛烷之反應基質上,具備過去文獻上從未有的位置與立體選擇性的專一性;此項研究展現了此酵素的活性中心內,高共軛性之紫質化合物或芳香族類之胺基酸與含氟取代基質間的交互作用,具備特定之弱作用力如凡得瓦作用力,能夠調整酵素的反應活性,包含轉換效率與催化選擇性;這些發現將為含氟化合物在藥物上的應用,提供重要的理論基礎。

原核生物之轉錄因子內鐵硫簇活性中心其感知氧化逆境與亞硝化逆境之結構與功能等研究
生物系統中,有一些金屬蛋白質含有鐵硫簇,他們能夠感應系統內之氧化還原環境的變化,驅使其可辨識之去氧核醣核酸片段,導向核醣核酸之轉錄活化;當相關的下游基因因此而啟動後,則得以表達適當的活化酵素,繼而除去生物體內高氧化性之因子或進行去氧核醣核酸的基因修補;這些活化因子的存在,主要的功能在於能夠感應到氧化逆境或氧分子的危害,也因此能夠讓生物體得以在含有氧分子的環境下生存,以獲取有效的能源利用。有趣的是,這些能夠感應有害的小分子含氧物質如過氧化物或氧分子的生物感知系統,也能夠與系統中的一氧化氮或一氧化氮的含鐵錯合物交互作用,轉換螯合成為具備順磁性質的金屬蛋白,而在電子順磁光譜儀中呈現g值平均值為2.03 的非均質訊號,然而,這些訊號極有可能來自於蛋白內,亞硝基鐵硫化物的生成;在這個研究中,我們期望能夠了解這些鐵硫簇蛋白是如何感應氣體小分子如一氧化氮或氧氣來防止或避免氧化逆境或亞硝化逆境對生物體內所造成的危害。

末端氧化酵素在大腸桿菌的全細胞催化系統



細胞色素P-450 BM-3等突變株高專一性地位置與立體選擇性的羥基化反應

大腸桿菌內轉錄因子在氧化或亞硝化逆境的調控



代表作
  1. Lo, F.-C.; Lee, J.-F.; Liaw, W.-F.; Hsu, I-J.; Chan, S. I.; Yu, S. S.-F. The Metal Core Structures in the Recombinant Escherichia coli Transcriptional Factor SoxR. Chem. Eur. J., 2012, 18, 2565.
  2. Maji, S.; Lee, J. C.-M.; Lu, Y.-J.; Chen, C.-L.; Hung, M.-C.; Chen, P. P.-Y.; Yu, S. S.-F.; Chan, S. I. Dioxygen Activation of a Trinuclear CuICuICuI Cluster Capable of Mediating Facile Oxidation of Organic Substrates: Competition between O-Atom Transfer and Abortive Inter-cluster Reduction. Chem. Eur. J. 2012, 3955.
  3. Wu, L.-L.; Yang, C.-L.; Lo, F.-C.; Chiang, C.-H.; Chang, C.-W.; Ng, K. Y.; Chou, H.-H.; Hung, H.-Y.; Chan, S. I.; Yu, S. S.-F. Tuning the Regioselectivity and Stereoselectivity in C-H Activation of n-Octanes by Cytochrome P450 BM-3 with Fluorine Substituents.  Evidence for Interactions Between a C-F Bond and Aromatic-pi Systems. Chem. Eur. J. 2011, 17, 4774.
  4. Ramu R.; Chang, C.-W.; Chou, H.-H.; Wu, L.-L.; Chiang, C.-H.; Yu, S. S.-F. Regio-selective Hydroxylation of gem-Difluorinated Octanes by Alkane Hydroxylase (AlkB). Tetrahedron Lett. 2011, 52(23), 2950-3.
  5. Chan S. I.; Nguyen H.-H. T.; Chen, K. H.-C.; Yu, S. S.-F. Over-expression and Purification of the Particulate Methane Monooxygenase from Methylococcus capsulatus (Bath). Methods Enzymol. 2011, 495, 177.
  6. Chan, S. I.; Yu S. S.-F. Controlled Oxidation of Hydrocarbons by the Membrane-bound Methane Monooxygenase: the Case for a Tricopper Cluster. Acc. Chem. Res. 2008, 41(8), 969.
  7. Ng, K.-Y.; Tu, L.-C.; Wang, Y.-S.; Chan, S.-I.; Yu, S. S.-F. Probing the Hydrophobic Pocket of the Active Site in the Particulate Methane Monooxygenase (pMMO) from Methylococcus capsulatus (bath) by Variable Stereoselective Alkane Hydroxylation and Olefin Epoxidation. ChemBioChem 2008, 9, 1116.
  8. Lo, F.-C.; Chen, C.-L.; Lee, C.-M., Tsai, M.-C., Lu, T.-T., Liaw, W.-F., Yu, S. S.-F. A Study of NO Trafficking from Dinitrosyl-iron Complexes to the Recombinant E. coli Transcriptional Factor SoxR. J. Biol. Inorg. Chem. 2008, 13, 961.
  9. Chan, S. I.; Wang, V. C.; Lai, J. C.; Yu, S. S.-F.; Chen, P. P.-Y.; Chen, K. K.-H.; Chen, C.-L.; Chan M.-K. Redox Potentiometry Studies of Particulate Methane Monooxygenase: Support for a Trinuclear Copper Cluster Active Site. Angew. Chem. Int., Ed. 2007, 46(12), 1992.
  10. Yu, S. S. F.; Ji, C.-Z.; Wu, Y.-P.; Lee, T.-L.; Lai, C.-H.; Lin, S.-C.; Yang, Z.-L.; Wang, V. C.-C.; Chen, K. H.-C.; Chan, S. I. The C-terminal Aqueous-exposed Domain of the 45 kDa Subunit of the Particulate Methane Monooxygenase in Methylococcus capsulatus (Bath) is a Cu(I) Sponge. Biochemistry 2007, 46, 13762.
  11. Tseng, C.-F.; Burger, A.; Mislin, G. L. A.; Schalk, I. J.; Yu, S. S.-F.; Chan, S. I.; Abdallah, M. A. Bacterial Siderophores: The Solution Stoichiometry and Coordination of the Fe(III) Complexes of Pyochelin and Related Compounds. J. Biol. Inorg. Chem. 2006, 11, 419.
  12. Chan, S. I.; Chen, K. H.-C.; Yu, S. S.-F.; Chen, C.-L.; Kuo, S. S.-J. Toward Delineating the Structure and Function of the Particulate Methane Monooxygenase (pMMO) from Methanotrophic Bacteria. Biochemistry 2004, 43, 4421.
  13. Yu, S. S.-F.; Chen, H.-C.; Luo,W.-I.; Huang, D.-S.; Chan, S. I. The Stereospecific Hydroxylation of [2,2-2H2]Butaneand Chiral Dideuteriobutanes by the Particulate Methane Monooxygenase from Methylococcus capsulatus (Bath). J. Biol. Chem. 2003, 278, 40658.
  14. Yu, S. S.-F.; Chen, H.-C.; Wang, Y.-S.; Tseng, C. F.; Huang, D.-S.; Chan, S. I. Production of High-Quality Particulate Methane Monooxygenase in High Yields from Methylococcus capsulatus (Bath) with a Hollow-Fiber Membrane Bioreactor. J. Bacteriol. 2003, 185, 5915.

Update: 2015-03-23

期刊論文
  1. Ramu Ravirala, Wanna Wondemagegn Hailemichael, Janmanchi Damodar, Tsai Yi-Fang, Liu Chih-Cheng, Mou Chung-Yuan, Yu Steve S.-F.* Mechanistic study for the selective oxidation of benzene and toluene catalyzed by Fe(ClO4)2 in an H2O2 -H2O-CH3CN system. Molecular Catalysis 2017-11441, 114-121.
  2. Tsai Yi-Fang, Luo Wen-I, Chang Jen-Lin, Chang Chun-Wei, Chuang Huai-Chun, Ramu Ravirala, Wei Guor-Tzo, Zen Jyh-Myng, Yu Steve S.-F.* Electrochemical Hydroxylation of C3–C12 n-Alkanes by Recombinant Alkane Hydroxylase (AlkB) and Rubredoxin-2 (AlkG) from Pseudomonas putida GPo1. Scientific Reports 2017-087(1), DOI: 10.1038/s41598-017-08610-w.
  3. Wang Vincent C.-C., Maji Suman, Chen Peter P.-Y., Lee Hung Kay, Yu Steve S.-F., Chan Sunney I.* Alkane Oxidation: Methane Monooxygenases, Related Enzymes, and Their Biomimetics. Chemical Reviews 2017-07117(13), 8574-8621.
  4. Yang Chung-Ling, Lin Cheng-Hung, Luo Wen-I, Lee Tsu-Lin, Ramu Ravirala, Ng Kok Yaoh, Tsai Yi-Fang, Wei Guor-Tzo, Yu Steve S.-F.* Mechanistic Study of the Stereoselective Hydroxylation of [2-2 H1 ,3-2 H1 ]Butanes Catalyzed by Cytochrome P450 BM3 Variants. Chemistry - A European Journal 2017-0223(11), 2571-2582.
  5. Chih-Cheng Liu, Ravirala Ramu, Sunney I. Chan*, Chung-Yuan Mou*, Steve S.-F. Yu* Chemistry in confined space: a strategy for selective oxidation of hydrocarbons with high catalytic efficiencies and conversion yields under ambient conditions. Catalysis Science & Technology 2016-09, 6. 7623-7630.
  6. Chih-Cheng Liu, Chung-Yuan Mou, Steve S.-F. Yu, Sunney I. Chan* Heterogeneous formulation of the tricopper complex for efficient catalytic conversion of methane into methanol at ambient temperature and pressure. Energy & Environmental Science 2016-019, 1361-1374.
  7. Minh D. Pham, Ya-Ping Lin, Quan Van Vuong, Penumaka Nagababu,m Brian T.-A. Chang, Kok Yaoh Ng, Chien-Hung Chen, Chau-Chung Han, Chung-Hsuan Chen, Mai Suan Li, Steve S.-F. Yu*, Sunney I. Chan* Inactivation of the particulate methane monooxygenase (pMMO) in Methylococcus capsulatus (Bath) by acetylene.. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS 20151854(12), 1842-52.
  8. Nai-Ti Lin, Kamani Satyanarayana, Chih-Hsien Chen, Yi-Fang Tsai, Steve S.-F. Yu, Sunney I. Chan, Tien-Yau Luh Controlling the Orientation of Pendants in Two-Dimensional Comb-Like Polymers by Varying Stiffness of Polymeric Backbones. MACROMOLECULES 2014-0947(18), 6166-6172.
  9. Yao-Sheng Chen, Wen-I Luo, Chung-Ling Yang, Yi-Jung Tu, Chun-Wei Chang, Chih-Hsiang Chiang, Chi-Yao Chang, Sunney I. Chan, Steve S.-F. Yu* Controlled Oxidation of Aliphatic C-H bonds in Metallo-Monooxygenases: Mechanistic Insights Derived from Studies on Deuterated and Fluorinated Hydrocarbons (Early Career Focused Review, Cover Art). JOURNAL OF INORGANIC BIOCHEMISTRY 2014-05134, 118-133.
  10. Penumaka Nagababu, Steve S.-F. Yu, Suman Maji, Ravirala Ramu and Sunney I. Chan* Developing an efficient catalyst for controlled oxidation of small alkanes under ambient conditions (cover art). CATALYSIS SCIENCE AND TECHNOLOGY 2014-044, 930-935.
  11. Wang DR, Hsiao JC, Wong CH, Li GC, Lin SC, Yu SSF, Chen W, Chang W, Tzou DL Vaccinia Viral Protein A27 is Anchored to the Viral Membrane via a Cooperative Interaction with Viral Membrane Protein A17.. JOURNAL OF BIOLOGICAL CHEMISTRY 2014-03289, 6639-6655.
  12. Peter P.-Y. Chen, Penumaka Nagababu, Steve S.-F. Yu, Sunney I. Chan* Development of the Tricopper Cluster as a Catalyst for the Efficient Conversion of Methane into MeOH. CHEMCATCHEM 2014-026, 429-437.
  13. Yao-Sheng Chen, Wen-I Luo, Tsu-Lin Lee, Steve S.-F. Yu*, Chi-Yao Chang* Identification of the proteins required for fatty acid desaturation in zebrafish (Danio rerio). BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 2013-11440(4), 671-6.
  14. Chih-Hsiang Chiang, Ravirala Ramu, Yi-Jung Tu, Chung-Ling Yang, Kok Yaoh Ng, Wen-I Luo, Charles H. Chen, Yu-Ying Lu, Chen-Lun Liu, Steve S.-F. Yu* Regioselective Hydroxylation of C12-C15 Fatty Acids with Fluorinated Substituents by Cytochrome P450 BM3 (highlighted as a frontispiece of the issue). CHEMISTRY-A EUROPEAN JOURNAL 2013-1019 (41),13680-13691.
  15. Sunney I. Chan*, Yu-Jhang Lu, Penumaka Nagababu, Suman Maji, Mu-Cheng Hung, Marianne M. Lee, I-Jui Hsu, Pham Dinh Minh, Jeff C.-H. Lai, Kok Yoah Ng, Sridevi Ramalingam, Steve S.-F. Yu*, Michael K. Chan* Efficient Oxidation of Methane to Methanol by Dioxygen Mediated by Tricopper Clusters. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 2013-0352, 3731-3735.
  16. Minh D. Pham, Steve S.-F. Yu, Chau-Chung Han*, Sunney I. Chan*  Improved mass spectrometric analysis of membrane proteins based on rapid and versatile sample preparation on nanodiamond particles.. ANALYTICAL CHEMISTRY 201385(14), 6748-55.
  17. Suman Maji, Jason C.-M. Lee, Yu-Jhang Lu, Chang-Li Chen, Mu-Cheng Hung, Peter P.-Y. Chen, Steve S.-F. Yu, Sunney I. Chan* Dioxygen Activation of a Trinuclear CuICuICuI Cluster Capable of Mediating Facile Oxidation of Organic Substrates: Competition between O-Atom Transfer and Abortive Inter-cluster Reduction. CHEMISTRY-A EUROPEAN JOURNAL 201218(13), 3955-3968.
  18. Penumaka Nagababu, Suman Maji, Manyam Praveen Kumar, Peter, P.-Y. Chen, Steve S.-F Yu*, Sunney I. Chan* Efficient room-temperature oxidation of hydrocarbons mediated by tricopper cluster complexes with different ligands. ADVANCED SYNTHESIS & CATALYSIS 2012354, 3275-3282.
  19. Feng-Chun Lo, Jyh-Fu Lee, Wen-Feng Liaw, I-Jui Hsu*, Yi-Fang Tsai, Sunney I. Chan, Steve S.-F. Yu* The Metal Core Structures in the Recombinant Escherichia coli Transcriptional Factor SoxR. CHEMISTRY-A EUROPEAN JOURNAL 201218(9), 2565-2577.
  20. Ravirala Ramu, Chun-Wei Chang, Ho-Husan Chou, Li-Lan Wu, Chih-Hsiang Chiang, Steve S.-F. Yu* Regio-selective hydroxylation of gem-difluorinated octanes by alkane hydroxylase (AlkB). TETRAHEDRON LETTERS 2011-0652(23), 2950-2953.
  21. Sunney I. Chan*, H.-Hoa T. Nguyen, Kelvin H.-C. Chen, Steve S.-F. Yu* OVER-EXPRESSION AND PURIFICATION OF THE PARTICULATE METHANE MONOOXYGENASE FROM METHYLOCOCCUS CAPSULATUS (BATH). Methods in Enzymology 2011-03495, 177-193.
  22. Li-Lan Wu, Chung-Ling Yang, Feng-Chun Lo, Chih-Hsiang Chiang, Chun-Wei Chang, Kok Yaoh Ng, Ho-Hsuan Chou, Huei-Ying Hung, Sunney I. Chan, Steve S.-F. Yu* Tuning the Regioselectivity and Stereoselectivity in C-H Activation of n-Octanes by Cytochrome P450 BM-3 with Fluorine Substituents. Evidence for Interactions Between a C-F Bond with Aromatic pi-Systems. CHEMISTRY-A EUROPEAN JOURNAL 201117, 4774-4787.
  23. Mansoureh Mousavi, SteveS.-F.Yu, Der-LiiM.Tzou A 13C solid-state NMR analysis of vitamin D compounds. SOLID STATE NUCLEAR MAGNETIC RESONANCE 200936, 24-31.
  24. Shih PC, Yang MS, Lin SC, Ho Y, Hsiao JC, Wang DR, Yu SS, Chang W, Tzou DL A turn-like structure "KKPE" segment mediates the specific binding of viral protein A27 to heparin and heparan sulfate on cell surfaces.. JOURNAL OF BIOLOGICAL CHEMISTRY 2009284(52), 36535-46.
  25. Lo F.-C., Chen C.-L., Lee C.-M., Tsai M.-C., Lu T.-T., Liaw W.-F., Yu S. S.-F.* A study of NO trafficking from dinitrosyl-iron complexes to the recombinant E. coli transcriptional factor SoxR.. JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY 200813(6), 961-72.
  26. Chan SI, Yu SS Controlled oxidation of hydrocarbons by the membrane-bound methane monooxygenase: the case for a tricopper cluster.. ACCOUNTS OF CHEMICAL RESEARCH 200841(8), 969-79.
  27. Kao WC, Wang VC, Huang YC, Yu SS, Chang TC, Chan SI Isolation, purification and characterization of hemerythrin from Methylococcus capsulatus (Bath).. JOURNAL OF INORGANIC BIOCHEMISTRY 2008102(8), 1607-14.
  28. Ng KY, Tu LC, Wang YS, Chan SI, Yu SS-F* Probing the hydrophobic pocket of the active site in the particulate methane monooxygenase (pMMO) from Methylococcus capsulatus (Bath) by variable stereoselective alkane hydroxylation and olefin epoxidation.. CHEMBIOCHEM 20089(7), 1116-23.
  29. Chan SI, Wang VC, Lai JC, Yu SS, Chen PP, Chen KH, Chen CL, Chan MK Redox potentiometry studies of particulate methane monooxygenase: support for a trinuclear copper cluster active site.. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 200746(12), 1992-4.
  30. Yu SS, Ji CZ, Wu YP, Lee TL, Lai CH, Lin SC, Yang ZL, Wang VC, Chen KH, Chan SI The C-terminal aqueous-exposed domain of the 45 kDa subunit of the particulate methane monooxygenase in Methylococcus capsulatus (Bath) is a Cu(I) sponge.. BIOCHEMISTRY 200746(48), 13762-74.
  31. Tseng, CF, Burger, A, Mislin, GLA, Schalk, IJ, Yu, SSF, Chan, SI and Abdallah, MA Bacterial siderophores: the solution stoichiometry and coordination of the Fe(III) complexes of pyochelin and related compounds. JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY 2006-0611(4), 419-432.
  32. M.S. Vinchurkar, K.H.C. Chen, S.S.F. Yu, S.J. Kuo, H.C. Chiu, S.H. Chien and S.I. Chan* Polarized ATR-FTIR spectroscopy of the membrane-embedded domains of the particulate methane monooxygenase.. Biochemistry 200443, 13283-13292.
  33. C.L. Chen, K.H.C. Chen, S.C. Ke, S.S.F. Yu and S.I. Chan* Preparation and characterization of a (Cu, Zn)-pMMO from Methylococcus capsulatus (Bath).. J. Inorg. Biochem. 200498, 2125-2130.
  34. Kao WC, Chen YR, Yi EC, Lee H, Tian Q, Wu KM, Tsai SF, Yu SS, Chen YJ, Aebersold R, Chan SI Quantitative proteomic analysis of metabolic regulation by copper ions in Methylococcus capsulatus (Bath).. J. Biol. Chem. 2004279(49), 51554-60.
  35. S.-C. Hung, C.-Li. Chen, K.H.-C. Chen, S.S.-F. Yu and S.I. Chan* The catalytic copper clusters of the particulate methane monooxygenase from methanotrophic bacteria: Electron paramagnetic resonance spectral simulations.. JOURNAL OF THE CHINESE CHEMICAL SOCIETY 200451, 1229-1244.
  36. K. H.-C. Chen, C.-L. Chen, C.-F. Tseng, S.S.-F. Yu, S.-C. Ke, J.-F. Lee, H.T. Nguyen, S.J. Elliott, J.O. Alben and S.I. Chan* The copper clusters in the particulate methane monooxygenase (pMMO) from Methylococcus capsulatus (Bath) .. JOURNAL OF THE CHINESE CHEMICAL SOCIETY 200451, 1081-1098.
  37. S.I. Chan*, K.H.C. Chen, S.S.F. Yu, C.L. Chen and S.S.J. Kuo Toward delineating the structure and function of the particulate methane monooxygenase from methanotrophic bacteria.. Biochemistry 200443, 4421-4430.
  38. S.S.F. Yu, L.Y. Wu, K.H.C. Chen, M.Y.H. Tseng, Y.S. Wang, C.F. Tseng, Y.J. Chen, D.S. Huang and S.I. Chan* Production of high quality pMMO in high yields from Methylococcus capsulatus (Bath) with a hollow-fiber membrane bioreactor.. J. Bacteriol. 2003185, 5915-5924.
  39. S.S.F. Yu, L.Y. Wu, K.H.C. Chen, W.I. Luo, D.S. Huang, S.I. Chan* The stereospecific hydroxylation of [2,2-2h2]butane and chiral dideuteriobutanes by the particulate methane monooxygenase from Methylococcus capsulatus (Bath).. J. Biol. Chem. 2003278, 40658-40669.
技術報告
  1. 俞聖法、羅鳳君、洪木成、廖文峰、蔡銘哲、魯才德,2009,〈生物系統中的鐵硫簇活性中心蛋白與亞硝基鐵硫錯合物〉,《化學》,第六十七卷第二期,頁133-141。。
  2. 李耀昌、陳俊榮、馮學深、李志甫、黃玉山、李明道、宋豔芳、鄭有舜、江素玉、陳慶曰、黃佩瑜、許益瑞、俞聖法、吳文桂,2008,〈同步輻射在生命科學上的應用與發展〉,《物理雙月刊》,第30卷第1期,頁8-26。
  3. Chan, K. H.-C.; Yu, S. S.-F.; Chan, S. I., 2005, “XAS Studies of pMMO from Methylococcus capsulatus (Bath)”, NSRRC Activity Report, pp. 65.
他類論文
  1. 2. 陳耀甥、張鈞崴、楊宗霖、吳麗嵐、江志祥、拉姆、羅文一、陳長謙、俞聖法,2013,〈金屬單加氧酵素內其活化烷烴基質選擇性的控制〉,《化學季刊》,第71卷第1期,頁67-81。