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鄒德里 (Der-Lii M. Tzou)

核磁共振光譜學、生物物理化學

鄒德里 (Der-Lii M. Tzou)

    886-2-55728524
    886-2-27831237
    tzougate*
副所長(102-103年);研究員(100年迄今);副研究員(94-100年);助研究員(88-94年);東海大學物理系畢業(69年);佛羅里達理工學院物理及天文學系碩士(76年);喬治亞理工學院物理博士(80年);德國馬克斯普朗克高分子研究所博士後研究(81-83年);中央研究院生物醫學科學研究所博士後研究(83-84年);中央研究院生物醫學科學研究所核磁共振及核磁造影實驗室特殊性技術人員(84-88年) ;
研究領域

著重痘病毒蛋白質間相互作用專一性以及蛋白質與細胞表面醣類間之相互作用專一性之研究。主題包括蛋白質本身自組裝形成多聚體之形成機制,以及和醣類之間結構與作用關係,藉由液態核磁共振及原二色分光儀分析蛋白質分子結構,配合電漿生物感應晶片分析病毒蛋白質間及病毒蛋白質與醣類相互作用關係,以期對病毒入侵正常細胞蛋白質分子結構與作用關係依存性作進一步之瞭解。另在固態核磁共振方面對雙交叉極化之三共振頻率實驗之機制進行研究,並對固醇類樣品作一系列分子結構分析鑑定。



代表作
  1. Li, G.-C.; Wang, D.-R.; Chen, W.; Tzou, D.-L.M. Solid-state NMR analysis of steroidal conformation of 17α- and 17β-estradiol in the absence and presence of lipid environment. Steroids 2012, 77, 185.
  2. Shih, P.-C.; Li, G.-C.; Yang, K.-J.; Chen, W.; Tzou, D.-L. Conformational analysis of steroid hormone molecules in the lipid environment - A solid-state NMR approach. Steroids 201176, 558.
  3. Ko, C.-H.; Chen, F.-J.; Lee, J.-J.; Tzou, D.-L.M. Effects of fiber physical and chemical characteristics on the interaction between endoglucanase and eucalypt fibers. Cellulose 201118, 1043.
  4. Cang, J.-R.; Lin, H.-M.; Cheng, S.-W.; Tseng, C.-K.; Tzou, D.-L.M.; Shyu, S.-G. EXAFS investigation of the morphology of immobilized Rh(PPh3)3Cl on phosphinated MCM-41. J. Mol. Catal. A: Chem. 2010329, 27.
  5. Shih, P.-C.; Yang, M.-S.; Lin, S.-C.; Yu, H.; Hsiao, J.-C.; Wang, D.-R.; Yu, S.-F.S.; Chang, W.; Tzou, D.-L.M. A turn-like structure “KKPE” segment mediates the specific binding of viral protein A27 to heparin and heparan sulfate on the cell surfaces. J. Biol. Chem. 2009, 284, 36535.
  6. Mousavi, M.; Yu, S.-F. S.; Tzou, D.-L. M. A 13C Solid-State NMR Analysis of Vitamin D Compounds. Solid State NMR 2009, 36, 24.
  7. Chang, S.-J.; Hsiao, J.-C.; Sonnberg, S.; Chiang, C.-T.; Yang, M.-H.; Tzou, D.-L. M.; Mercer, A. A.; Chang, W. Poxviral Host Range Protein CP77 Contains a F-box Like Domain that is Necessary for Suppression NF-kB Signaling but is Independent of its Host Range Function. J. Virol. 2009, 89, 4140.
  8. Liu, W.-T.; Lin, S.-C.; Chou, W.-I.; Liu, T.-H.; Pan, R.-L.; Tzou, D.-L. M.; Hua, T.-E.; Chang, M. D.-T. Identification and Characterization of a Novel Fibril Forming Peptide in Fungal Starch Binding Domain. Biochem. Biophys. Res. Commun. 2008, 377, 966.
  9. Yang, J.-H.; Chou, F.-C.;Tzou, D.-L. M. Coherence Selection in double CP MAS NMR spectroscopy. J. Magn. Reson. 2008, 195, 116.
  10. Yang, J.-H.; Ho, Y.; Tzou, D.-L. M. A 13C solid-state NMR analysis of steroid compounds. Magn. Reson. Chem. 2008, 46, 718.
  11. Wasielewski, E.; Tzou, D.-L. M.; Dillmann, B.; Abdallah, M. A.; Atkinson, R. A.; Kieffer, B. Multiple Conformations of the Metal-Bound Pyoverdine PvdI, a Siderophore of Pseudomonas aeruginosa: a Nuclear Magnetic Resonance Study. Biochemistry 2008, 47, 3397.
  12. Chu, F.-I.; Ho, Y.; Huang, C.-J.; Tzou, D.-L. 1H, 13C and 15N resonance assignments for pro-apoptotic protein Nix (residue 1-156) from Danio rerio. Biomol. NMR Assign. 2007, 1, 29.
  13. Chang, C.-W; Chen, Y.-N.; Adak, A. K.; Lin, K.-H.; Tzou, D.-L.; Lin, C.-C. Synthesis of phytosphingosine using olefin cross-metathesis: a convenient access to chain-modified phytosphingosines from D-lyxose. Tetrahedron 2007, 63, 4310.
  14. Chiu, W.-L.; Lin, C.-L.; Yang, M.-H.; Tzou, D.-L.; Chang, W. Vaccinia virus 4c(A26L) protein on intracellular mature virus binds to the extracellular cellular matrix laminin. J. Virol. 2007, 81, 2149.
  15. Yang, H.; Lin, S. M.; Lin, Y.H.; Wang, Y.; Chen, M. M.; Sheu, H. S.; Tzou, D.-L. M.; Lin, C. H.; Luh, T. Y. Ring opening metathesis polymerization of bisnorbornene derivatives linked by Cp2Ni2(m-S)2 bridge.. J. Organomet. Chem. 2006, 691, 3196.
  16. Ho, Y.; Chu, F.-I.; Tzou, D.-L. M. Solid-state NMR studies of the molecular structure of Taxol. Magn. Reson. Chem. 2006, 44, 581.
  17. Tzou, D.-L. M.; Wasielewski, E.; Abdallah, M. A.; Kieffer, B.; AtkinsonA low temperature, R. A. heteronuclear NMR study of two exchanging conformations of metal-bound pyoverdin PaA from Pseudomonas aeruginosa. Biopolymers 2005, 79, 139.
  18. Chu, F.-I.; Ho, Y.; Tzou, D.-L. M. Letter to the Editor: NMR assignment of the vaccinia virus envelope protein A27L. J. Biomol. NMR 2005, 32, 178.
  19. Ho, Y.; Hsiao, J.-C.; Peng, Y.-C.; Yang, M.-H.; Chung, C.-S.; Lin, T.-H.; Chang, W.; Tzou, D.-L. M. The oligomeric structure of vaccinia viral envelope protein A27L is essential for binding to heparin and heparan sulfates on cell surface: A structural and functional approach using site-specific mutagenesis. J. Mol. Biol. 2005, 349, 1060.
  20. Tzou, D.-L. M. A solid-state NMR application of the anomeric effect in carbohydrates: galactosamine, glucosamine, and N-acetyl-glucosamine. Solid State NMR 2005, 27, 209.
  21. Chen, Y.-y.; Luo, S.-Y.; Hung, S.-C.; Chan, S. I.; Tzou, D.-L. M. 13C solid-state NMR chemical shift anisotropy analysis of the anomeric carbon in carbohydrates. Carbohydr. Res. 2005, 340, 723.
  22. Lin, C.-C.; Pan, Y.-S.; Patkar, L. N.; Lin, H.-M.; Tzou, D.-L. M.; Subramanianb, T.; Lin, C.-C. Versatile Approach for the Synthesis of Novel Seven-membered Iminocyclitols via Ring-closing Metathesis Dihydroxylation Reaction. Bioorg. & Med. Chem. 2004, 12, 3259.
  23. Chiu, H.-Y.; Tzou, D.-L. M.; Patkar, L. N.; Lin, C.-C. A Facile Synthesis of Phytosphingosine from Mannofuranoside. J. Org. Chem. 2003, 68, 5788.
  24. Lin, T.-H.; Chia, C.-M.; Hsiao, J.-C.; Chang, W.; Ku, C.-C.; Hung, S.-C.; Tzou, D.-L. M. Structural Analysis of the Extracellular Domain of Vaccinia Virus Envelope Protein, A27L, by NMR and CD Spectroscopy. J. Biol. Chem. 2002, 277, 20949.
  25. Su, T.-W.; Tzou, D.-L. M. Solid-state Fast Magic Angle Spinning 19F NMR Studies of Morphology in Poly(vinylidene fluoride). Polymer, 2000, 41, 7289.

Update: 2015-03-23

期刊論文
  1. Chang JY, Carollo KD, Lin SC, Wu YY, Tzou DM NMR investigation of magnesium chelation and cation-induced signal shift effect of testosterone.. Steroids 2016-11115, 18-25.
  2. Yi-Hsin Chen, Hsin-Kuan Liu, Wen-Jung Chang, Der-Lii Tzou, Kwang-Hwa Lii* High-Temperature, High-Pressure Hydrothermal Synthesis, Characterization, and Structural Relationships of Mixed-Alkali Metals Uranyl Silicates. JOURNAL OF SOLID STATE CHEMISTRY 2016-04236, 55-60.
  3. Kathleen J. Carillo, Susan Arco, Cheng-Chung Wang, Der-Lii M. Tzou* Solid-State NMR Investigation of Effect of Fluorination and Methylation on Prednisolone Conformation Steroids. STEROIDS 2015-12104, 263-269.
  4. Rey Y. Capangpangan, Mira Anne C. dela Rosa, Rofeamor P. Obena, Yu-Jen Chou, Der-Lii Tzou, Shao-Ju Shih, Ming-Hsi Chiang, Chun-Cheng Lin*, Yu-Ju Chen* Monodispersity of Magnetic Immuno-Nanoprobes Enhances the Detection Sensitivity of Low Abundance Biomarkers in One Drop of Serum. ANALYST 2015-10140, 7678-7686.
  5. Darong Wang, Meiman Chen, Rong-Jie Chein, Wei-Min Ching, Chen-Hsiung Hung, Der-Lii M. Tzou* Cation Ion Specifically Induces a Conformational Change in Trans-Dehydroandrosterone - A Solid-State NMR Study. STEROIDS 2015-0496, 73-80.
  6. Yu-Sheng Wang, Chun-Han Ko, Hao-Ting Chang, Kai-Jay Yang, Yu-Jen Chen, Shing-Jong Huang, Pei-Ju Fang, Chi-Fon Chang, Der-Lii M. Tzou* 1H, 13C and 15N Backbone and Side-Chain Resonance Assignments of A Family 36 Carbohydrate Binding Module of Xylanase from Paenibacillus Campinasensis. Biomolecular NMR Assignments 2014-108, 303-306.
  7. Da-Rong Wang, Jye-Chian Hsiao, Chien-Hsuan Wong, Guo-Chian Li, Su-Ching Lin, Steve S.-F. Yu, Wenlung Chen, Wen Chang, Der-Lii M. Tzou* Vaccinia Viral Protein A27 is Anchored to the Viral Membrane via a Cooperative Interaction with Viral Membrane Protein A17. JOURNAL OF BIOLOGICAL CHEMISTRY 2014-03289, 6639-6655.
  8. Kai-Jay Yang, Su-Ching Lin, Shing-Jong Huang, Wei-Min Ching, Chen-Hsiung Hung, Der-Lii M. Tzou* Solid-State NMR Study of Fluorinated Steroids. STEROIDS 2014-0280, 64-70.
  9. Der-Lii M. Tzou*, Lin-Kai Ni, Mei-Man Chen, Min-Chuan Chiou, Li-Chou Chen, Shan-Te Hsu, Kuo-Lung Ku, and Chien-Chung Cheng* Fingerprints of Phalaenopsis Tissues in Growth and Spike Induction Periods-A Solid-state 13C NMR Approach. JOURNAL OF THE CHINESE CHEMICAL SOCIETY 2013-09, 60(9)1107-1112.
  10. Chian-Hui Lai, Tsung-Che Chang, Yung-Jen Chuang, Der-Lii Tzou, Chun-Cheng Lin* Stepwise Orthogonal Click Chemistry toward Fabrication of Paclitaxel/Galactose Functionalized Fluorescent Nanoparticles for HepG2 cell Targeting and Delivery. BIOCONJUGATE CHEMISTRY 2013-0824(10), 1698-1709.
  11. Der-Lii M. Tzou* Bandwidth in double cross-polarization MAS NMR spectroscopy. SOLID STATE NUCLEAR MAGNETIC RESONANCE 2012-1047, 23-27.
  12. Guo-Chian Li, Da-Rong Wang, Wenlung Chen, Der-Lii M. Tzou* Solid-State NMR Analysis of Steroidal Conformation of 17α- and 17β-Estradiol in the Absence and Presence of Lipid Environment. STEROIDS 2012-0277,185-192.
  13. Ping-Chen Shih, Guo-Chian Li, Kai-Jay Yang, Wenlung Chen, Der-Lii M. Tzou* Conformational Analysis of Steroid Hormone Molecules in the Lipid Environment - A Solid-State NMR Approach. STEROIDS 2011-0576(6), 558-63.
  14. Chun-Han Ko*, Fang-Jing Chen, Jey Jau Lee, Der-Lii M. Tzou Effects of Fiber Physical and Chemical Characteristics on the Interaction Between Endoglucanase and Eucalypt Fibers. CELLULOSE 2011-0418(4), 1043-1054.
  15. Jen-Ray Chang, Hsiu-Mei Lin, Sheau-Wen Cheng, Chia-Kai Tseng, Der-Lii Tzou, Shin-Guang Shyu* EXAFS Investigation of the Morphology of Immobilized Rh(PPh3)3Cl on Phosphinated MCM-41. JOURNAL OF MOLECULAR CATALYSIS A-CHEMICAL 2010-09329, 27-35.
  16. Shih, Ping-Chen Shih, Min-Shiang Yang, Su-Ching Lin, Yu Ho, Jye-Chian Hsiao, Da-Rong Wang, Steve S.-F. Yu, Wen Chang, Der-Lii M. Tzou* A Turn-Like Structure “KKPE” Segment Mediates the Specific Binding of Viral Protein A27 to Heparin and Heparan Sulfate on the Cell Surfaces. JOURNAL OF BIOLOGICAL CHEMISTRY 2009-12284,36535-36546.
  17. Mansoureh Mousavi, Steve S.-F.Yu, Der-Lii M.Tzou* A (13)C Solid-State NMR Analysis of Vitamin D Compounds. SOLID STATE NUCLEAR MAGNETIC RESONANCE 2009-0936, 24-31.
  18. Virginie Ropars, Philippe Barthe, Chi-Shien Wang, Wenlung Chen, Der-Lii M. Tzou, Anne Descours, Loïc Martin, Masayuki Noguchi, Daniel Auguin, Christian Roumestand* Structural Studies of the Complex Between Akt-in and the Akt2-PH Domain Suggest that the Peptide Acts as an Allosteric Inhibitor of the Akt Kinase. THE OPEN SPECTROSCOPY JOURNAL 2009-073, 65-76.
  19. Shu-Jung Chang, Jye-Chian Hsiao, Stephanie Sonnberg, Cheng-Ting Chiang, Min-Hsiang Yang, Der-Lii Tzou, Andrew A. Mercer, Wen Chang* Poxvirus Host Range Protein CP77 Contains an F-Box-Like Domain That Is Necessary To Suppress NF-kB Activation by Tumor Necrosis Factor Alpha but Is Independent of Its Host Range Function. JOURNAL OF VIROLOGY 2009-0583, 4140-4152.
  20. Wei-Ting Liu, Shu-Chuan Lin, Wei-I Chou, Tseng-Huang Liu, Rong-Long Pan, Der-Lii Tzou, Tzu-En Hua, Margaret Dah-Tsyr Chang* Identification and Characterization of a Novel Fibril Forming Peptide in Fungal Starch Binding Domain. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 2008-12377, 966-970.
  21. Jen-Hsien Yang, Fang-Chieh Chou, Der-Lii M. Tzou* Coherence Selection in Double CP MAS NMR Spectroscopy. JOURNAL OF MAGNETIC RESONANCE 2008-11195, 116-120.
  22. Jen-Hsien Yang, Yu Ho, Der-Lii M. Tzou* A 13C Solid-State NMR Analysis of Steroid Compounds . MAGNETIC RESONANCE IN CHEMISTRY 2008-0846, 718-725.
  23. Emeric Wasielewski, Der-Lii Tzou, Baudoin Dillmann, Jerzy Czaplicki,Mohamed A. Abdallah, R. Andrew Atkinson, Bruno Kieffer* Multiple Conformations of the Metal-Bound Pyoverdine PvdI, a Siderophore of Pseudomonas Aeruginosa: a Nuclear Magnetic Resonance Study. BIOCHEMISTRY 2008-0347, 3397-3406.
  24. Chi-We Chang, Yeng-Nan Chen, Avijit Kumar Adak, Kun-Hsien Lin, Der-Lii M. Tzou, Chun-Cheng Lin* Synthesis of Phytosphingosine Using Olefin Cross-Metathesis: A Convenient Access to Chain-Modified Phytosphingosines from D-Lyxose. TETRAHEDRON 2007-05 63, 4310-4318.
  25. Feng-I Chu, Yu Ho, Chang-Jen Huang, Der-Lii M. Tzou* 1H, 13C and 15N Resonance Assignments for Proapoptotic Protein Nix (Residue 1~156) from Danio Rerio. Biomolecular NMR Assignments 2007-051, 29-31.
  26. Wen-Ling Chiu, Chi-Long Lin, Min-Hsiang Yang, Der-Lii M. Tzou, Wen Chang* Vaccinia Virus 4c (A26L) Protein on Intracellular Mature Virus Binds to the Extracellular Cellular Matrix Laminin. JOURNAL OF VIROLOGY 2007-0381, 2149-2157.
  27. Hui-Chun Yang, Shiu-Mei Lin, Yi-Hung Liu, Yu Wang, Mei-Man Chen, Hwo-Shuenn Sheu, Der-Lii Tsou, Chih-Hsiu Lin, Tien-Yau Luh* Ring Opening Metathesis Polymerization of Bisnorbornene Derivatives Linked by Cp2Ni2(μ-S)2 Bridge. JOURNAL OF ORGANOMETALLIC CHEMISTRY 2006-07691, 3196-3200.
  28. Yu Ho, Der-Lii M. Tzou*, Feng-I Chu Solid-State NMR Studies of the Molecular Structure of Taxol. MAGNETIC RESONANCE IN CHEMISTRY 2006-0644(6), 581-585.
  29. Der-Lii Tzou, Emeric Wasielewski, Mohamed A. Abdallah, Bruno Kieffer*, R. Andrew Atkinson A Low-Temperature Heteronuclear NMR Study of Two Exchanging Conformations of Metal-Bound Pyoverdin PaA from Pseudomonas Aeruginosa. BIOPOLYMERS 2005-1079, 139-149.
  30. Der-Lii M. Tzou* A Solid-State NMR Application of the Anomeric Effect in Carbohydrates: Galac Tosamine, Glucosamine, and N-Acetyl-Glucosamine. SOLID STATE NUCLEAR MAGNETIC RESONANCE 2005-0627, 209-214.
  31. Yu Ho, Jye-Chian Hsiao, Min-Hsiang Yang, Che-Sheng Chung, Yu-Chang Peng, Ta-Hsien Lin, Wen Chang*, Der-Lii M. Tzou* The Oligomeric Structure of Vaccinia Viral Fusion Protein A27L is Essential for Binding to Heparin and Heparan Sulfates on Cell Surface: A Structural and Functional Approach Using Site-Specific Mutagenesis. JOURNAL OF MOLECULAR BIOLOGY 2005-06349, 1060-1071.
  32. Feng-I Chu, Yu Ho, Der-Lii M. Tzou* Letter to the Editor: NMR Assignment of the Vaccinia Virus Envelope Protein A27L. JOURNAL OF BIOMOLECULAR NMR 2005-0632, 178.
  33. Yu Ho, Der-Lii M. Tzou* Coil-Coiled Motifs in Proteins. CHEMISTRY (The Chinese Chemical Society, Taipei) 2005-0663(2), 269-280.
  34. Der-Lii M. Tzou* Solid-State NMR Characterization of Monosaccharides. CHEMISTRY (The Chinese Chemical Society, Taipei) 2005-0663(2), 185-192.
  35. Ying-Ying Chen, Shun-Yuan Luo, Shang-Cheng Hung, Sunney I. Chan, Der-Lii M. Tzou* 13C Solid-State NMR Chemical Shift Anisotropy Analysis of Anomeric Carbon in Carbohydrates. CARBOHYDRATE RESEARCH 2005-03340, 723-729.
  36. Chang-Ching Lin, Yi-shin Pan, Laxmikant N. Patkar, Hsiu-Mei Lin, Der-Lii M. Tzou, Thangaiah Subramanian, Chun-Cheng Lin* Versatile Approach for the Synthesis of Novel Seven-Membered Iminocyclitols via Ring-closing Metathesis Sihydroxylation Reaction. BIOORGANIC & MEDICINAL CHEMISTRY 2004-0612, 3259-3267.
  37. Hsin-Yi Chiu, Der-Lii M. Tzou*, Laxmikant Narhari Patkar, Chun-Cheng Lin* A Facile Synthesis of Phytosphingosine from Diisopropylidene-D-Mannofuranose. JOURNAL OF ORGANIC CHEMISTRY 2003-0768, 5788-5791.
  38. Ta-Hsien Lin, Chih-Ming Chia, Jye-Chian Hsiao, Wen Chang, Chiao-Chu Ku, Shang-Cheng Hung, Der-Lii M. Tzou* Structural Analysis of the Extracellular Domain of Vaccinia Virus Envelope Protein, A27L, by NMR and CD Spectroscopy. JOURNAL OF BIOLOGICAL CHEMISTRY 2002-06277, 20949-20959.
  39. Tsao-Wen Su, Der-Lii M. Tzou* Solid-state Fast Magic Angle Spinning 19F NMR Studies of Morphology in Poly(vinylidene fluoride). POLYMER 2000-0941, 7289-7293.
  40. Shu-Ming Lee, Der-Lii Tzou, George J. Y. Chiou, Hong N. Yeung* An Alternative Scheme for 13C Chemical Shift Imaging via Inverse Detection of Proton through a MILS (or Inverse SLIM) Technique. JOURNAL OF MAGNETIC RESONANCE 1998-01130(1), 102-110.
  41. Der-Lii Tzou, Shu-Ming Lee, Hong N. Yeung* Temperature Dependence and Phase Transition of Proton Relaxation of Hydrated Collagen in Intact Beef Tendon via Cross-Relaxation. MAGNETIC RESONANCE IN MEDICINE 1997-0337(3), 359-365.
  42. Hong N. Yeung, Der-Lii Tzou, SM Lee, Fu-Yung Huang, Y Hur Indirect Estimation of Lens Protein Dynamics via Magnetization Transfer. JOURNAL OF MAGNETIC RESONANCE SERIES B 1996-11113(2),167-171.