Chang, Wei-Hau

Single Molecule Biophysics, Single Particle Cryo-Electron Microscopy, Chemical Biology for Bio-Conjugation

Chang, Wei-Hau (章為皓)

    886-2-27898558
    886-2-27831237
    weihau
Associate Research Fellow (2012-);Assistant Fellow (2003-2012);Adjunct Assistant Fellow of Genomic Research Center (2004-);Adjunct Assistant Professor of Biochemical Technology National Taiwan University (2009-);B.S. National Taiwan University (1989);M.S. University of California Berkeley (1993);Ph.D. Stanford University (2001);Postdoctoral Fellow, Stanford University (2001-2003) ;
Research Interests

My laboratory is interested in elucidating the structure and function of biological macromolecules in yeast and human cells, especially these molecules governing the transfer of genetic information, such as RNA polymerase I, II, III, DNA repair enzymes, splicing particles, and various kinds of infectious virus such as influenza and EV71. How these macromolecules interact with their partners and how these molecules change their conformations when they act upon their targets? These questions have to be answered by a combination of various techniques. To this end, our lab is developing chemical methods to label these macromolecules in vitro and also in vivo and aiming to image them with single molecule microscopy, such as FRET, PALM, and cryo electron microscopy. We are also applying yeast genetics and chemical synthesis to design novel bio-conjugation strategies, and developing new image analysis algorithm for analyzing single particle electron microscope images. We welcome students and postdocs with the background of chemistry, biology, physics, mathematics, and electric engineering to join our venture at this frontier.





Selected Publications
  1. Yi-min Wu, Chun-hsiung Wang, Yi-yun Chen, Naoyuki Miyazaki, Kazuyoshi Murata, Kuniaki Nagayama, Wei-Hau Chang 2013 Zernike phase contrast cryo-electron microscopy reveals 100 kDa component in a protein complex, J. Phys. D: Appl. Phys. 46, 494008 (special issue on phase contrast)
  2. Jen-wei Chang, Yi-min Wu, Zi-Yun Chen, Shih-hsin Huang, Chun-hsiung Wang, Pei-lun Wu, Yi-ping Weng, Changjiang You, Jacob Piehler, Wei-hau Chang 2013 Hybrid Electron Microscopy-FRET imaging localizes the dynamical C-terminus of Tfg2 in RNA polymerase II-TFIIF with nanometer precision , Journal of Structural Biology 184, 52-62 (special issue on hybrid methods)
  3. Yi-min Wu, Jen-wei Chang, Chun-hsiung Wang,Yen-chen Lin, Pei-lun Wu, Shih-hsin Huang, Chia-chi Chang, Xiaopeng Hu, Averell Gnatt*, Wei-hau Chang 2012 Regulation of mammalian transcription by Gdown1 through a novel steric crosstalk revealed by cryo-electron microscopy, EMBO JOURNAL 31, 3575-3587
  4. Wei-hau Chang, Michael T.-K. Chiu, Chin-Yu Chen, Chi.-Fu Yen, Yen-Cheng Lin, Holland Cheng, Jianhua Fu, I-ping Tu 2010 Zernike Phase plate cryo-electron microscopy facilitates single particle analysis of asymmetric proteins, Structure 18, 17-27
  5. Chin-Yu Chen, Chia-Chi Chang, Chi-fu Yen, Michael T.-K. Chiu, Wei-hau Chang (2009) Mapping RNA exit on RNA polymerase II by FRET analysis, PNAS 106, 127-132

Update: 2015-03-23

Journal Paper
  1. Chen Ting-Li, Hsieh Dai-Ni, Hung Hung, Tu I-Ping, Wu Pei-Shien, Wu Yi-Ming, Chang Wei-Hau, Huang Su-Yun $gamma$-SUP: A clustering algorithm for cryo-electron microscopy images of asymmetric particles. The Annals of Applied Statistics 20148(1), 259-285.
  2. Yi-min Wu, Chu-Hsiung Wang, Jen-wei Chang, Kazuyoshi Murata, Kuniaki Nagayama, Wei-hau Chang* Zernike phase contrast cryo-electron microscopy reveals 100 kDa component in a protein complex. JOURNAL OF PHYSICS D-APPLIED PHYSICS 2013-1246(49), 494008-494017.
  3. Jen-Wei Chang, Yi-Min Wu, Zi-Yun Chen, Shih-Hsin Huang, Chun-Hsiung Wang, Pei-lun Wu, Yi-ping Weng, Changjiang You, Jacob Piehler, Wei-hau Chang* Hybrid electron microscopy-FRET imaging localizes the dynamical C-terminus of Tfg2 in RNA polymerase II–TFIIF with nanometer precision. JOURNAL OF STRUCTURAL BIOLOGY 2013-07184(1), 52-62.
  4. Yi-min Wu, Jen-wei Chang, Chun-hsiung Wang,Yen-chen Lin, Pei-lun Wu, Shih-hsin Huang, Chia-chi Chang, Xiaopeng Hu, Averell Gnatt*, Wei-hau Chang * Regulation of mammalian transcription by Gdown1 through a novel steric crosstalk revealed by cryo-electron microscopy. EMBO JOURNAL 2012-0831(17), 3575-3587.
  5. Hou-chih Lee, Bo-lin Lin, Wei-hau Chang *, I-ping Tu* Toward automated de-noising of single-molecule FRET data: ADN for smFRET. JOURNAL OF BIOMEDICAL OPTICS 2012-0217, 011007.
  6. Jiahn-Haur Liao, Chiao-I Kuo, Ya-Yi Huang, Yu-Ching Lin, Yen-Chen Lin, Chen-Yui Yang, Wan-Ling Wu, Wei-Hau Chang, Yen-Chywan Liaw, Li-Hua Lin, Chung-I Chang, Shih-Hsiung Wu* A Lon-Like Protease with No ATP-Powered Unfolding Activity. PLOS ONE 2012, PLoS ONE 7(7): e40226.
  7. Hung-Ju Wei , Weihau Chang , Shih-Chang Lin , Wen-Chun Liu , Ding-Kao Chang , Pele Chong , Suh-Chin Wu* Fabrication of influenza virus-like particles using M2 fusion proteins for imaging single viruses and designing vaccines. Vaccine 2011-0929, 7163-7172.
  8. CC Fu, CF Lin, QZe Gao, WZ Yang, TS Lim,LL Yang, CF Yen, Wei-Hau Chang, Hanna S. Yuan d, S-Y Sheu,Dah-Yen Yang, Wunshain Fann Fis-protein induces rod-like DNA bending. Chemical Physics Letters 2010-10500,318-322.
  9. Wang CH, Hsu CH, Wu YM, Luo YC, Tu MH, Chang WH, Cheng RH, Lin CS* Roles of cysteines Cys115 and Cys201 in the assembly and thermostability of grouper betanodavirus particles. VIRUS GENES 2010-0841, 73-80.
  10. Yen-Chen Lin, Bo-lin Lin, Tommy Setiawan, Chia-Chi Cheng, Chi-Fu Yen, Wei-hau Chang* A single molecule FRET study of formation of RNA polymerase II elongation complex on passivated surface . Journal of Chinese Chemical Society 2010-0757(3B), 514-521.
  11. Wei-hau Chang, Yu-hua Liu Bioorthogonal labeling method for single molecule FRET. Journal of Chinese Chemical Society 2010-0757(3B),505-513.
  12. Wei-hau Chang, Michael T.-K. Chiu, Chin-Yu Chen, Chi-Fu Yen, Yen-Cheng Lin, Yi-ping Weng, Ji-chau Chang, Yi-min Wu, Holland Cheng, Jianhua Fu, I-ping Tu Zernike phase plate cryo-electron microscopy facilitates single particle analysis of unstained asymmetric protein complexes. Structure 2010-0118, 17-27.
  13. Velusamy M, Huang JH, Hsu YC, Chou HH, Ho KC, Wu PL, Chang WH, Lin JT, Chu CW* Dibenzo[f,h]thieno[3,4-b] quinoxaline-based small molecules for efficient bulk-heterojunction solar cells. Org. Lett. 2009-1111,4898-4901.
  14. Chen CY, Chang CC, Yen CF, Chiu MT, *Chang WH Mapping RNA exit channel on transcribing RNA polymerase II by FRET analysis.. Proceedings of the National Academy of Sciences of the United States of America 2009106(1), 127-32.
  15. Jiessie Shiue, Chia-Seng Chang, Sen-Hui Huang, Chih-Hao Hsu, Jin-Sheng Tsai, Wei-hau Chang, Yi-Min Wu, Yen-Chen Lin, Pai-Chia Kuo, Yan-Shan Huang, Yeukuang Hwu, Ji-Jung Lai, Fang-Gang Tseng, and Fu-Rong Chen Phase TEM for biological imaging utilizing a Boersch elecrostatic phase plate: theory and practice. Journal of Electron Microscopy 200958,137-145.
  16. Yi-Min Wu, Chi-Hsin Hsu, Chun-Hsiung Wang, Wangta Liu, Wei-hau Chang* and Chan-Shing Lin* Role of DxxDxD motif in the assembly and stability of the betanodavirus particles. ARCHIVES OF VIROLOGY 2008-08, 153:1633-1642.
  17. Chang WH, Kornberg RD Electron crystal structure of the transcription factor and DNA repair complex, core TFIIH.. Cell 2000102(5), 609-13.