中央研究院化學研究所

我們實驗室主要用生物物理工具和化學生物手段探討控制人類遺傳訊息傳遞和修復的蛋白質巨分子。目前我們專注的課題包括：核醣核酸聚合酶與它的轉錄因子所形成的複合結構，核醣核酸聚合酶被泛素修飾的機制，流感和手口足病毒結構的解析和侵入細胞的機理。我們使用的工具有低溫電子顯微鏡影像重建，單分子螢光能共振顯微術，和酵母菌分子生物學。電子顯微鏡可以幫助我們得到次奈米的結構，而螢光顯微鏡可以幫助我們得到這些分子的動態。為了有效從事電子顯微鏡和螢光顯微鏡造影，我們也用化學生物共軛方法把奈米金和染劑接到蛋白質上。我們歡迎具有合成化學、生物學、物理學、數學和電機工程背景的年輕人參與我們的團隊，一起作好玩的實驗。

• 李氏基金會  (2004)

1. Chung, S.-c.; Lin, H.-H.; Niu, B.-Y.; Huang, S.-H.; Tu, I-P.; Chang, W.-H. Pre-Pro is a fast pre-processor for single particle cryo-EM by enhancing 2D classification. Commun. Biol. 2020, Accepted.
2. Huang, C.-F.; Chang, W.-H.; Lee, T.-K.; Joti, Y.; Nishino, Y.; Kimura, T.; Suzuki, A.; Bessho, Y.; Lee, T.-T.; Chen, M.-C.; Yang, S.-M.; Hwu, Y.; Huang, S.-H.; Li, P.-N.; Chen, P.; Tseng, Y.-C.; Ma, C.; Hsu, T.-L.; Wong, C.-H.; Tono, K.; Ishikawa, T.; Liang, K. S. XFEL coherent diffraction imaging for weakly scattering particles using heterodyne interference. AIP Adv. 2020, 10 (5), 055219.
3. Štěrbová, P.; Wu, D.; Lou, Y.-C.; Wang, C.-H.; Chang, W.-H.; Tzou, D.-L. M. NMR assignments of protrusion domain of capsid protein from dragon grouper nervous necrosis virus. Biomol NMR Assign 2019, 14, 63-66.
4. Wu, J.-S.; Chen, T.-Y.; Lin, S. S.-Y.; Lin, S.-Y.; Hung, C.-Y.; Tu, I-P.; Chen, H.-T.; Chang, W.-H. Deriving a sub-nanomolar affinity peptide from TAP to enable smFRET analysis of RNA polymerase II complexes. Methods 2019, 159-160, 59-69.
5. Wu, Y.-M.; Wang, C.-H.; Chen, Y.-Y.; Miyazaki, N.; Murata, K.; Nagayama, K.; Chang, W.-H. Zernike phase contrast cryo-electron microscopy reveals 100 kDa component in a protein complex, J. Phys. D: Appl. Phys. 2013, 46, 494008 (special issue on phase contrast)
6. Chang, J.-W.; Wu, Y.-M.; Chen, Z.-Y.; Huang, S.-H.; Wang, C.-H.; Wu, P.-l.; Weng, Y.-P.; You, C.; Piehler, J.; Chang, W.-H. Hybrid Electron Microscopy-FRET imaging localizes the dynamical C-terminus of Tfg2 in RNA polymerase II-TFIIF with nanometer precision. J. Struct. Biol. 2013, 184, 52-62 (special issue on hybrid methods)
7. Wu, Y.-M.; Chang, J.-W.; Wang, C.-H.; Lin, Y.-C.;Wu, P.-L.; Huang, S.-H.; Chang, C.-C.; Hu, X.; Gnatt, A.; Chang, W.-H. Regulation of mammalian transcription by Gdown1 through a novel steric crosstalk revealed by cryo-electron microscopy, EMBO J. 2012, 31, 3575-3587
8. Chang, W.-H.; Chiu, M. T.-K.; Chen, C.-Y.; Yen, C.-F.; Lin, Y.-C.; Cheng, H.; Fu, J.; Tu, I-P. Zernike Phase plate cryo-electron microscopy facilitates single particle analysis of asymmetric proteins, Structure 2010, 18, 17-27
9. Chen, C.-Y.; Chang, C.-C.; Yen, C.-F.; Chiu, M. T.-K.; Chang, W.-H. Mapping RNA exit on RNA polymerase II by FRET analysis, PNAS 2009, 106, 127-132

Update： 2020-08-20

1. Szu-chi Chung, Hsin-Hung Lin, Bo-Yao Niu, Shih-Hsin Huang, I-Ping Tu, Wei-hau Chang* Pre-Pro is a fast pre-processor for single particle cryo-EM by enhancing 2D classification. Communications Biology 2020-08, (Accepted).
2. Szu-chi Chung, Shao-hsuan Wang, Bo-yao Niu, Su-yun Huang, Wei-hau Chang, I-Ping Tu* Two-stage dimension reduction (2SDR) for noisy high dimensional images and application to cryogenic electron microscopy. Annals of Mathematical Sciences and Applications 2020-08, (Accepted).
3. Chi-Feng Huang, Wei-Hau Chang*, Ting-Kuo Lee, Yasumasa Joti, Yoshinori Nishino, Takashi Kimura, Akihiro Suzuki, Yoshitaka Bessho, Tsung-Tse Lee, Mei-Chun Chen, Shun-Min Yang, Yeukuang Hwu, Shih-Hsin Huang, Po-Nan Li, Peilin Chen, Yung-Chieh Tseng, Che Ma, Tsui-Ling Hsu, Chi-Huey Wong, Kensuke Tono, Tetsuya Ishikawa, Keng S. Liang. XFEL coherent diffraction imaging for weakly scattering particles using heterodyne interference. AIP Advances 2020-05, 10(5), 055219.
4. Petra Štěrbová, Danni Wu, Yuan-Chao Lou, Chun-Hsiung Wang, Wei-Hau Chang*, Der-Lii M Tzou*. NMR assignments of protrusion domain of capsid protein from dragon grouper nervous necrosis virus. Biomolecular NMR Assignments 2020-04, 14(1), 63-66.
5. Jheng-Syong Wu, Tzu-Yun Chen, Sam Song-Yao Lin, Shu-Yu Lin, Cheng-Yu Hung, I-Ping Tu, Hung-Ta Chen, Wei-Hau Chang* Deriving a sub-nanomolar affinity peptide from TAP to enable smFRET analysis of RNA polymerase II complexes. METHODS 2019-02, pii: S1046-2023(18)30301-3. doi: 10.1016/j.ymeth.2.
6. Huang Chi-Feng, Liang Keng S., Hsu Tsui-Ling, Lee Tsung-Tse, Chen Yi-Yun, Yang Shun-Min, Chen Hsiang-Hsin, Huang Shih-Hsin, Chang Wei-Hau, Lee Ting-Kuo, Chen Peilin, Peng Kuei-En, Chen Chien-Chun, Shi Cheng-Zhi, Hu Yu-Fang, Margaritondo Giorgio, Ishikawa Tetsuya, Wong Chi-Huey, Hwu Y. Free-electron-laser coherent diffraction images of individual drug-carrying liposome particles in solution. Nanoscale 2018, 10(6), 2820-2824.
7. Min-Yuan Chia, Wan-Yu Chung, Chung-Hsiung Wang, Wei-Hau Chang, Min-Shi Lee* Development of A High-Growth Enterovirus 71 Vaccine Candidate Inducing Cross-Reactive Neutralizing Antibody Responses. VACCINE 2018, 21;36(9):1167-1173.
8. Wong HC, Wang TY, Yang CW, Tang CT, Ying C, Wang CH, Chang WH Characterization of a lytic vibriophage VP06 of Vibrio parahaemolyticus.. Research in microbiology 2018, pii: S0923-2508(18)30117-7.
9. Chung-Shu Yeh, Shang-Lin Chang, Jui­-Hui Chen, Hsuan-Kai Wang, Yue-Chang Chou, Chun-Hsiung Wang, Shih-Hsin Huang, Amy Larson, Jeffrey A Pleiss, Wei-Hau Chang, Tien-Hsien Chang* The conserved AU dinucleotide at the 5′ end of nascent U1 snRNA is optimized for the interaction with nuclear cap-binding-complex. Nucleic Acids Research 2017, 45(16), 9679-9693.
10. Ting-Li Chen*, Dai-Ni Hsieh*, Hung Hung, I-Ping Tu*, Pei-Shien Wu, Yi-Ming Wu*, Wei-Hau Chang*, Su-Yun Huang* $gamma$-SUP: A clustering algorithm for cryo-electron microscopy images of asymmetric particles. The Annals of Applied Statistics 2014, 8(1), 259-285.
11. Yi-min Wu, Chu-Hsiung Wang, Jen-wei Chang, Kazuyoshi Murata, Kuniaki Nagayama, Wei-hau Chang* Zernike phase contrast cryo-electron microscopy reveals 100 kDa component in a protein complex. JOURNAL OF PHYSICS D-APPLIED PHYSICS 2013-12, 46(49), 494008-494017.
12. Jen-Wei Chang, Yi-Min Wu, Zi-Yun Chen, Shih-Hsin Huang, Chun-Hsiung Wang, Pei-lun Wu, Yi-ping Weng, Changjiang You, Jacob Piehler, Wei-hau Chang* Hybrid electron microscopy-FRET imaging localizes the dynamical C-terminus of Tfg2 in RNA polymerase II–TFIIF with nanometer precision. JOURNAL OF STRUCTURAL BIOLOGY 2013-07, 184(1), 52-62.
13. Yi-min Wu, Jen-wei Chang, Chun-hsiung Wang,Yen-chen Lin, Pei-lun Wu, Shih-hsin Huang, Chia-chi Chang, Xiaopeng Hu, Averell Gnatt*, Wei-hau Chang * Regulation of mammalian transcription by Gdown1 through a novel steric crosstalk revealed by cryo-electron microscopy. EMBO JOURNAL 2012-08, 31(17), 3575-3587.
14. Hou-chih Lee, Bo-lin Lin, Wei-hau Chang *, I-ping Tu* Toward automated de-noising of single-molecule FRET data: ADN for smFRET. JOURNAL OF BIOMEDICAL OPTICS 2012-02, 17, 011007.
15. Jiahn-Haur Liao, Chiao-I Kuo, Ya-Yi Huang, Yu-Ching Lin, Yen-Chen Lin, Chen-Yui Yang, Wan-Ling Wu, Wei-Hau Chang, Yen-Chywan Liaw, Li-Hua Lin, Chung-I Chang, Shih-Hsiung Wu* A Lon-Like Protease with No ATP-Powered Unfolding Activity. PLOS ONE 2012, PLoS ONE 7(7): e40226.
16. Hung-Ju Wei , Weihau Chang , Shih-Chang Lin , Wen-Chun Liu , Ding-Kao Chang , Pele Chong , Suh-Chin Wu* Fabrication of influenza virus-like particles using M2 fusion proteins for imaging single viruses and designing vaccines. Vaccine 2011-09, 29, 7163-7172.
17. CC Fu, CF Lin, QZe Gao, WZ Yang, TS Lim,LL Yang, CF Yen, Wei-Hau Chang, Hanna S. Yuan d, S-Y Sheu,Dah-Yen Yang, Wunshain Fann Fis-protein induces rod-like DNA bending. Chemical Physics Letters 2010-10, 500,318-322.
18. Wang CH, Hsu CH, Wu YM, Luo YC, Tu MH, Chang WH, Cheng RH, Lin CS* Roles of cysteines Cys115 and Cys201 in the assembly and thermostability of grouper betanodavirus particles. VIRUS GENES 2010-08, 41, 73-80.
19. Yen-Chen Lin, Bo-lin Lin, Tommy Setiawan, Chia-Chi Cheng, Chi-Fu Yen, Wei-hau Chang* A single molecule FRET study of formation of RNA polymerase II elongation complex on passivated surface . Journal of Chinese Chemical Society 2010-07, 57(3B), 514-521.
20. Wei-hau Chang, Yu-hua Liu Bioorthogonal labeling method for single molecule FRET. Journal of Chinese Chemical Society 2010-07, 57(3B),505-513.
21. Wei-hau Chang, Michael T.-K. Chiu, Chin-Yu Chen, Chi-Fu Yen, Yen-Cheng Lin, Yi-ping Weng, Ji-chau Chang, Yi-min Wu, Holland Cheng, Jianhua Fu, I-ping Tu Zernike phase plate cryo-electron microscopy facilitates single particle analysis of unstained asymmetric protein complexes. Structure 2010-01, 18, 17-27.
22. Velusamy M, Huang JH, Hsu YC, Chou HH, Ho KC, Wu PL, Chang WH, Lin JT, Chu CW* Dibenzo[f,h]thieno[3,4-b] quinoxaline-based small molecules for efficient bulk-heterojunction solar cells. Org. Lett. 2009-11, 11,4898-4901.
23. Jiessie Shiue, Chia-Seng Chang, Sen-Hui Huang, Chih-Hao Hsu, Jin-Sheng Tsai, Wei-hau Chang, Yi-Min Wu, Yen-Chen Lin, Pai-Chia Kuo, Yan-Shan Huang, Yeukuang Hwu, Ji-Jung Lai, Fang-Gang Tseng, and Fu-Rong Chen Phase TEM for biological imaging utilizing a Boersch elecrostatic phase plate: theory and practice. Journal of Electron Microscopy 2009, 58,137-145.
24. Chen CY, Chang CC, Yen CF, Chiu MT, *Chang WH Mapping RNA exit channel on transcribing RNA polymerase II by FRET analysis.. Proceedings of the National Academy of Sciences of the United States of America 2009, 106(1), 127-32.
25. Yi-Min Wu, Chi-Hsin Hsu, Chun-Hsiung Wang, Wangta Liu, Wei-hau Chang* and Chan-Shing Lin* Role of DxxDxD motif in the assembly and stability of the betanodavirus particles. ARCHIVES OF VIROLOGY 2008-08, 153:1633-1642.
26. Chang WH, Kornberg RD Electron crystal structure of the transcription factor and DNA repair complex, core TFIIH.. Cell 2000, 102(5), 609-13.