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Institute of Chemistry, Academia Sinica – People

Surface Physical and Materials Chemistry, Chemical and Systems Biology

Hsiung-Lin Tu (涂熊林)

    886-2-55728622
    886-2-27831237
    hltu gate.sinica.edu.tw

Hsiung-Lin Tu's Lab
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Associate Research Fellow (2024-)；Assistant Research Fellow (2017 - 2024)； EMBO Long-Term Postdoctoral Fellow, University of Chicago (2016-2017) and ETH Zurich (2014-2016)； Postdoctoral researcher, QB3 University of California Berkeley (2013-2014)； PhD, University of California Berkeley (2013)； MS, National Taiwan University (2005)； BS, National Chiao Tung University (2003). ；

Muneer, G.; Gebreyesus, S.; Chen, C.-S.; Lee, T.-T.; Yu, F.; Lin, C.-A.; Hsieh, M.-S.; Nesvizhskii, A.; Ho, C.-C.; Yu, S.-L.; Tu, H.-L.*; Chen, Y.-Ju. Mapping Nanoscale-To-Single-Cell Phosphoproteomic Landscape by Chip-DIA. Adv. Sci. 2025, 12(1), 2402421.
Montalbo, R.; Wu, M.-J.; Tu, H.-L.* One-step flow synthesis of size-controlled polymer nanogels in a fluorocarbon microfluidic chip. RSC Adv. 2024, 14(16), 11258-11265.
Cheng, C.-T.; Hsiao, J.-C.; Hoffmann, A.; Tu, H.-L.* TNFR1 mediates heterogeneity in single-cell NF-κB activation. iScience 2024, 27(4), 109486.
Rakesh, N.; Tu, H.-L.; Chang, P.-C.; Gebreyesus, S.; Lin, C.-J.* Innovative real-time flow sensor using detergent-free fluorescent complex emulsions with dual-emissive semi-perfluoroalkyl substituted α-cyanostilbene. Adv. Sci. 2023, 2304108.
Li, Y.; Chang, C.-C.; Wang, C.; Wu, W.-T.; Wang, C.-M.*; Tu, H.-L.* Microfluidic biosensor decorated with an indium phosphate nanointerface for attomolar dopamine detection. ACS Sensors 2023, 8, 2263-70. (Supplementary Cover).
Chen, Y.-C.; Li, Y.; Yan, S. C.-C.; Hsu, C.-P.; Cheng, P.-L.*; Tu, H.-L.* DNA tension assays reveal that force-dependent integrin activation regulates neurite outgrowth in primary cortical neurons. Biomater. Adv. 2023, 150, 213431.
Gebreyesus, S. T.; Muneer, G.; Huang, I. C.-C.; Siyal, A.; Anand, M.; Chen, Y.-J.; Tu, H.-L.* Recent advances in microfluidics for single-cell functional proteomics. Lab Chip 2023, 23, 1726-51.
Montalbo, R.; Tu, H.-L.* Micropatterning of functional lipid membrane for quantitative bioanalysis. Biomicrofluidics 2023, 17, 031302.
Yin, M.-C.; Wei, P.-C.; Li, Y.; Hsu, T.; Chang, K.-C.; Lu, C.-P.; Tu, H.-L.; Wang, C.-M.* Structural variants and ultra-low detection ability for tryptamine in two polymorphs of a zincophosphate framework. Inorg. Chem. 2023, 62(44), 18150-56.
Herianto, S.; Chien, P.-J.; Ho, J.-A.; Tu, H.-L.* Liposome-based artificial cells: from gene expression to reconstitution of cellular functions and phenotypes. Biomater. Adv. 2022, 142, 213156.
Gebreyesus, S. T.; Siyal, A.; Kitata, R.; Chen, E. S.-W.; Enkhbayar, B.; Angata, T.; Lin, K.-I.; Chen, Y.-J.; Tu, H.-L.* Streamlined single-cell proteomics by an integrated microfluidic chip and data-independent acquisition mass spectrometry. Nat. Commun. 2022, 13, 37.
Chien, P.-J.; Shih, Y.-L.; Cheng, C.-T.; Tu, H.-L.* Chip-assisted formation of phase-separated liposomes for reconstituting spatial protein-lipid interactions. Lab Chip 2022, 22, 2540-48.
Kao, S.-H.; Liang, S.-Y.; Cheng, P.-L.; Tu, H.-L.* Surface viscosity-dependent neurite initiation in cortical neurons. Adv. Biol. 2022, 6, 2101325.
Ti, Y.-T.; Cheng, H.-C.; Li, Y.; Tu, H.-L.* Multiplexed patterning of hybrid lipid membrane and protein arrays for cell signaling study. Lab Chip 2021, 21, 2711-2720. (Back Cover).
Son, M.; Wang, A.; Tu, H.-L.; Metzig, M.; Patel, P.; Husain, K.; Lin, J.; Murugan, A.; Hoffmann, A.; Tay, S. NF-κB responds to absolute differences in cytokine concentrations. Sci. Signal. 2021, 14, eaaz4382.
Li, Y.; Xie, J.-F.; Chang, C.-C.; Wang, C.-M.*; Tu, H.-L.* Highly sensitive detection of mercury ion using zincophosphite framework nanoparticle-polyaniline composites. ACS Appl. Nano Mater. 2020, 3, 9724. (Supplementary Cover).
Zhang, C.; Tu, H.-L.; Jia, G.; Mukhtar, T.; Taylor, V.; Rzhtsky, A.; Tay, S. Ultra-Multiplexed Analysis of Single Cell Dynamics Reveals Logic Rules in Differentiation. Sci. Adv. 2019, 5, eaav7959.
Christensen, S.; Tu, H.-L.; Jun, J.; et al. One-way membrane trafficking of SOS in receptor-triggered Ras activation. Nat. Struct. Mol. Biol. 2016, 23, 838-846.
Iversen, L.; Tu, H.-L.; et al. Ras activation by SOS: Allosteric regulation by altered fluctuation dynamics. Science 2014, 345, 50-54.
Lin, W.-C.; Iversen, L.; Tu, H.-L.; et al. H-Ras forms dimers on membrane surfaces via a protein-protein interface. Proc. Natl. Acad. Sci. 2014, 111, 2996-3001.
Tu, H.-L.; Lin, Y.-S.; Lin, H.-Y.; Hung, Y.; Lo, L.-W.; Chen, Y.-F.; Mou, C.-Y. In-vitro studies of functionalized mesoporous silica nanoparticles for photodynamic therapy. Adv. Mater. 2009, 21, 172-177.
Tu, H.-L.; Tsai, F.-Y.; Mou, C.-Y.; et al. One step synthesis of ordered mesostructural organic/silica nanocomposite with tunable fluorescence surfactants. J. Mater. Chem. 2008, 18, 1771-1778.

Update： 2025-01-16

Samuel Herianto and Hsiung-Lin Tu* Recent insights into bacterial ESCRT-III-mediated membrane remodeling. Critical Reviews in Biochemistry and Molecular Biology 2026-02, 61, 1–19.
Meng-Jie Wu, Reynaldo Montalbo, Chun-Jen Su, Orion Shih and Hsiung-Lin Tu* One-step photochemical synthesis of gold-polymer nanohybrids with engineered morphologies and catalytic activity. Nanoscale 2026-01, .
K.-H. Lyu, J.-W. Chen, D.-Y. Jin, Y.-J. Huang, H.-L. Tu, A. K Adak* and Chun-Cheng Lin* Boronate-mediated covalent and oriented immobilization of antibodies on the PDMS surface toward improved capture of circulating tumor cells. Lab on a Chip 2025-12, 26, 211-219.
S. Herianto, H.-M. Lee, C. Chen, J.-A. Ho, and H.-L. Tu* Functional reconstitution of bacterial ESCRT-III protein PspA identifies key regions in membrane binding and remodeling. Protein Science 2025-09, 34(10), e70309.
K. Devi, M. Ganesan, S.-M. Chen, Y. Li, H.-L. Tu, C.-M. Wang, Y.-F. Duann, G. Gopu Graphitic carbon nitride-embedded MXene tungsten carbide nanoflakes for sensitive detection of cytotoxic tinidazole in biological samples. Journal of the Taiwan Institute of Chemical Engineers 2025-06, 171, 106072.
S. Ali, Y.-L. Chen, H.-S. Tseng, H. Ayalew, J.-W. She, B. Gautam, H.-L. Tu*, Y.-S. Hsiao*, and H.-H. Yu* Poly(3,4-ethylenedioxythiophene) Nanorod Arrays-Based Organic Electrochemical Transistor for SARS-CoV-2 Spike Protein Detection in Artificial Saliva. ACS Sensors 2025-03, 10(3), 2007-2018.
J.-F. Xie, P.-C. Wei, Y. Li, C.-C. Chang, K.-C. Chang, C.-P. Lu, T. Hsu, D.-L. Tzou, H.-L. Tu*, and C.-M. Wang* Unique Organic–Inorganic Hybrid Copper(I) Phosphate with Ultralow Ractopamine Detection Limit and In Situ Sensing Ability. Inorganic Chemistry 2025-02, 64(9), 4408-4414.
Gul Muneer, Sofani Gebreyesus, Ciao‐Syuan Chen, Tzu‐Tsung Lee, Fengchao Yu, Chih‐An Lin, M.‐S. Hsieh, A. I. Nesvizhskii, Chao‐Chi Ho, Sung‐Liang Yu, Hsiung‐Lin Tu*, and Yu‐Ju Chen* Mapping Nanoscale‐To‐Single‐Cell Phosphoproteomic Landscape by Chip‐DIA. Advanced Science 2025-01, 2025, 12(1).
Fei-Ting Hsu*, Ying-Tzu Chen, Yu-Cheng Chin, Li-Chan Chang, Shu-Chin Chiang, Li-Xing Yang, Hua-Shan Liu, Po-Fu Yueh, Hsiung-Lin Tu, Ruei-Yu He, Long-Bin Jeng, Woei-Cheang Shyu, Shang-Hsiu Hu, I-Tsang Chiang, Yu-Chang Liu, Yi-Chun Chiu, Guan-Chun Wu, Ching-Ching Yu, Wen-Pin Su*, and Chih-Chia Huang* Harnessing the Power of Sugar-Based Nanoparticles: A Drug-Free Approach to Enhance Immune Checkpoint Inhibition against Glioblastoma and Pancreatic Cancer. ACS Nano 2024-10, 18, 28764-28781.
Subburaj Srinithi, Naveen Karuppusamy, S.M. Chen*, S. Balaji Natarajan, Balakumar Vellaichamy*, Ying Li, Hsiung-Lin Tu*, Chih-Min Wang*, and Yeh-Fang Duann* Ultra-sensitive zinc cobaltate assembled on N-rich carbon nitride electrochemical sensor for the detection of paraquat in food samples. Journal of the Taiwan Institute of Chemical Engineers 2024-10, 165, 105779.
Reynaldo C. Montalbo, Meng-Jie Wu and Hsiung-Lin Tu* One-step flow synthesis of size-controlled polymer nanogels in a fluorocarbon microfluidic chip. RSC Advances 2024-04, 14(16), 11258-11265.
Chieh-Teng Cheng, Jye-Chian Hsiao, Alexander Hoffmann and Hsiung-Lin Tu* TNFR1 mediates heterogeneity in single-cell NF-κB activation. iScience 2024-04, 27 (4), 109486.
Mu-Chien Yin, Pi-Chen Wei, Ying Li, Todd Hsu, Jia-Yi Jian, Kai-Chi Chang, Ching-Ping Lu, Hsiung-Lin Tu*, Chih-Min Wang* Structural Variants and Ultralow Detection Ability for Tryptamine in Two Polymorphs of a Zincophosphite Framework. Inorganic Chemistry 2023-11, 62(44), 18150-18156.
Narani Rakesh†, Hsiung‐Lin Tu†, Po‐Chun Chang, Sofani T. Gebreyesus, Che‐Jen Lin* Innovative real‐time flow sensor using detergent‐free complex emulsions with dual‐emissive semi‐perfluoroalkyl substituted <i>Α</i>‐cyanostilbene. Advanced Science 2023-11, 10(31), 2304108.
Ying-Chi Chen, Ying Li, Ching-Cher S. Yan, Chao-Ping Hsu, Pei-Lin Cheng* and Hsiung-Lin Tu* DNA tension assays reveal that force-dependent integrin activation regulates neurite outgrowth in primary cortical neurons. Biomaterials Advances 2023-07, 150, 213431.
Ying Li, Chiao-Chun Chang, Chu Wang, Wen-Ti Wu, Chih-Min Wang* and Hsiung-Lin Tu* Microfluidic biosensor decorated with an indium phosphate nanointerface for attomolar dopamine detection. ACS Sensors 2023-06, 8(6), 2263-2270.
Reynaldo Carlos K. Montalbo and Hsiung-Lin Tu* Micropatterning of functional lipid bilayer assays for quantitative bioanalysis (Invited Perspective). Biomicrofluidics 2023-05, 17(3), 031302.
Sofani Gebreyesus, Gul Muneer, Chih-Cheng Huang, Asad Siyal, Mihir Anand, Yu-Ju Chen and Hsiung-Lin Tu* Recent advances in microfluidics for single-cell functional proteomics. Lab on a Chip 2023-04, 23, 1726-1751.
Samuel Herianto, Po-Jen Chien, Ja-an Annie Ho and Hsiung-Lin Tu* Liposome-based artificial cells: From gene expression to reconstitution of cellular functions and phenotypes. Biomaterials Advances 2022-11, 142, 213156.
Chen Chen, Chien-Hsin Chu, Ying Chu, Ting-Ya Chang, Sheng-Wen Chen, Shu-Yang Liang, Yun-Chi Tsai, Bi-Chang Chen, Hsiung-Lin Tu and Pei-Lin Cheng* Neuronal paxillin and Drebrin mediate BDNF-induced force transduction and growth cone turning in a soft tissue-like environment. Cell Reports 2022-08, 40 (7), 111188.
Po-Jen Chien, Yi-Lun Shih, Chieh-Teng Cheng and Hsiung-Lin Tu* Chip assisted formation of phase-separated liposomes for reconstituting spatial protein–lipid interactions. Lab on a Chip 2022-06, 22, 2540-2548.
Shih-Han Kao, Shu-Yang Liang, Pei-Lin Cheng and Hsiung-Lin Tu* Surface viscosity-dependent neurite initiation in cortical neurons. Advanced Biology 2022-06, 6 (6), 2101325.
Sofani Gebreyesus, Asad Siyal, Reta Kitata, Eric Sheng-Wen Chen, Bayarmaa Enkhbayar, Takashi Angata, Kuo-I Lin, Yu-Ju Chen* and Hsiung-Lin Tu* Streamlined single-cell proteomics by an integrated microfluidic chip and data-independent acquisition mass spectrometry. Nature Communications 2022-01, 13, 37.
Asad Siyal, Eric Sheng-Wen Chen, Hsin-Ju Chan, Reta Birhanu Kitata, Jhih-Ci Yang, Hsiung-Lin Tu and Yu-Ju Chen* Sample Size-Comparable Spectral Library Enhances Data-Independent Acquisition-Based Proteome Coverage of Low-Input Cells. Analytical Chemistry 2021-12, 93 (51), 17003-17011.
Chu Wang , Jia-Rong Li, Ying Li, Hsiung-Lin Tu, Der-Lii M Tzou and Chih-Min Wang* Stable Crystalline Organic-Inorganic Hybrid Indium Phosphate with Dye Removal and Ractopamine Detection Applications. Inorganic Chemistry 2021-07, 60 (15), 11655-11660.
Yu-Ting Ti, Hsiao-Chi Cheng, Ying Li and Hsiung-Lin Tu* Multiplexed patterning of hybrid lipid membrane and protein arrays for cell signaling study. Lab on a Chip 2021-07, 21, 2711-2720.
Da-Wei Lin, Yang Liu, Yue-Qi Lee, Po-Jiun Yang, Chia-Tse Ho, Jui-Chung Hong, Jye-Chian Hsiao, Der-Chien Liao, An-Jou Liang, Tzu-Chiao Hung, Yu-Chuan Chen, Hsiung-Lin Tu, Chao-Ping Hsu and Hsiao-Chun Huang* Construction of intracellular asymmetry and asymmetric division in Escherichia coli. Nature Communications 2021-02, 12, 888.
Minjun Son, Andrew Wang, Hsiung-Lin Tu, Marie Metzig, Parthiv Patel, Kabir Husain, Jing Lin, Arvind Murugan, Alexander Hoffmann and Savaş Tay* NF-κB responds to absolute differences in cytokine concentrations. Science Signaling 2021-01, 14 (666), eaaz4382.
Ying Li, Ji-Fang Xie, Chiao-Chun Chang, Chih-Min Wang * and Hsiung-Lin Tu* Highly Sensitive Detection of Mercury Ions Using Zincophosphite Framework Nanoparticle-Polyaniline Composites. ACS Applied Nano Materials 2020-09, 3 (10), 9724-9730.
Jui Hsieh, Ju-Ying Chen, Han-Ying Li, Hsin-Kuan Liu, Hsiung-Lin Tu and Chih-Min Wang* A thio-functionalized zinc phosphite with a large-channel framework and enhanced removal ability of mercury ion from aqueous solutions. Dalton Transactions 2020-06, 49 (32), 11085-11089.
Udesh Dhawan, Wei‐Li Wang, Bhaskarchand Gautam, Jayakrishnan Janardhanan, Po‐Chiang Hsiao, Hsiung‐Lin Tu and Hsiao‐Hua Yu* Mechanotactic Activation of TGF‐β by PEDOT Artificial Microenvironments Triggers Epithelial to Mesenchymal Transition. Advanced Biosystems 2020-01, 4 (2), 1900165.
Jing Lin, Christian Jordi, Minjun Son, Hoang Van Phan, Nir Drayman, Mustafa Abasiyanik, Luke Vistain, Hsiung-Lin Tu and Savaş Tay* Ultra-sensitive digital quantification of proteins and mRNA in single cells. Nature Communications 2019-08, 10(1), 3544.
Ce Zhang, Hsiung-Lin Tu, Gengjie Jia, Tanzila Mukhtar, Verdon Taylor, Andrey Rzhetsky and Savaş Tay* Ultra-multiplexed analysis of single-cell dynamics reveals logic rules in differentiation. Science Advances 2019-04, 5(4), eaav7959.
S. Christensen, H.-L. Tu, J. Jun, S. Alvarez, M. Triplet, J. Iwig, K. Yadav, D. Bar-Sagi, J. Roose and J. Groves One-way Membrane Trafficking of SOS in Receptor-Triggered Ras Activation. Nature Structural & Molecular Biology 2016, 23, 838-846.
W. Y.-C. Huang, Q. Yan, W.-C. Lin, J. Chung, S. Hansen, S. Christensen, H.-L. Tu, J. Kuriyan and J. Groves Phosphotyrosine-mediated LAT Assembly on Membranes Drives Kinetic Bifurcation in Recruitment Dynamics of The Ras Activator SOS. Proceedings of the National Academy of Sciences USA 2016, 113, 8218-8223.
S. Christensen, M. Triplet, C. Rhodes, J. Iwig, H.-L. Tu, D. Stamou and J. Groves Monitoring The Waiting Time Sequence of Single Ras GTPase Activation Events Using Liposome Functionalized Zero Mode Waveguides. Nano Letters 2016, 16, 2890-2895.
Lars Iversen, Hsiung-Lin Tu, Wan-Chen Lin, Sune M. Christensen, Steven M. Abel, Jeff Iwig, Hung-Jen Wu, Jodi Gureasko, Christopher Rhodes, Rebecca S. Petit, Scott D. Hansen, Peter Thill, Cheng-Han Yu, Dimitrios Stamou, Arup K. Chakraborty, John Kuriyan, Ras Activation by SOS: Allosteric Regulation by Altered Fluctuation Dynamics. SCIENCE 2014, 345, 50-54.
Wan-Chen Lin, Lars Iversen, Hsiung-Lin Tu, Christopher Rhodes, Sune M. Christensen, Jeffrey S. Iwig, Scott D. Hansen, William Y. C. Huang, Jay T. Groves H-Ras Forms Dimers on Membrane Surfaces via A Protein-Protein Interface. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2014, 111, 2996-3001.
T. Lohmüller, L. Iversen, M. Schmidt, C. Rhodes, Hsiung-Lin Tu, W.-C. Lin, J. T. Groves Single Molecule Tracking on Supported Membranes with Arrays of Optical Nanoantennas. NANO LETTERS 2012, 12, 1717-1721.
Hsiung-Lin Tu, Yu-Shen Lin, Hsia-Yu Lin, Yann Hung, Leu-Wei Lo, Yang-Fang Chen, Chung-Yuan Mou In-vitro Studies of Functionalized Mesoporous Silica Nanoparticles for Photodynamic Therapy. ADVANCED MATERIALS 2009, 21, 172-177.
Hsiung-Lin Tu, Fu-Yu Tsai, C.-Y. Mou One Step Synthesis of Ordered Mesostructural Organic/silica Nanocomposite with Tunable Fluorescence Surfactants. JOURNAL OF MATERIALS CHEMISTRY 2008, 18, 1771-1778.
Fu-Yu Tsai, Hsiung-Lin Tu, Chung-Yuan Mou. Nonionic Fluorescent Oligomeric Surfactant for Ordered Mesoporous Silica Structure. JOURNAL OF MATERIALS CHEMISTRY 2006, 16, 348-350.