Institute of Chemistry, Academia Sinica--Hsiung-Lin Tu

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Surface Physical and Materials Chemistry, Chemical and Systems Biology

Hsiung-Lin Tu (涂熊林)

    886-2-55728622
    886-2-27831237
    hltu gate.sinica.edu.tw

Hsiung-Lin Tu's Lab
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Associate Research Fellow (2024-)；Assistant Research Fellow (2017 - 2024)； EMBO Long-Term Postdoctoral Fellow, University of Chicago (2016-2017) and ETH Zurich (2014-2016)； Postdoctoral researcher, QB3 University of California Berkeley (2013-2014)； PhD, University of California Berkeley (2013)； MS, National Taiwan University (2005)； BS, National Chiao Tung University (2003). ；

Muneer, G.; Gebreyesus, S.; Chen, C.-S.; Lee, T.-T.; Yu, F.; Lin, C.-A.; Hsieh, M.-S.; Nesvizhskii, A.; Ho, C.-C.; Yu, S.-L.; Tu, H.-L.*; Chen, Y.-Ju. Mapping Nanoscale-To-Single-Cell Phosphoproteomic Landscape by Chip-DIA. Adv. Sci. 2025, 12(1), 2402421.
Montalbo, R.; Wu, M.-J.; Tu, H.-L.* One-step flow synthesis of size-controlled polymer nanogels in a fluorocarbon microfluidic chip. RSC Adv. 2024, 14(16), 11258-11265.
Cheng, C.-T.; Hsiao, J.-C.; Hoffmann, A.; Tu, H.-L.* TNFR1 mediates heterogeneity in single-cell NF-κB activation. iScience 2024, 27(4), 109486.
Rakesh, N.; Tu, H.-L.; Chang, P.-C.; Gebreyesus, S.; Lin, C.-J.* Innovative real-time flow sensor using detergent-free fluorescent complex emulsions with dual-emissive semi-perfluoroalkyl substituted α-cyanostilbene. Adv. Sci. 2023, 2304108.
Li, Y.; Chang, C.-C.; Wang, C.; Wu, W.-T.; Wang, C.-M.*; Tu, H.-L.* Microfluidic biosensor decorated with an indium phosphate nanointerface for attomolar dopamine detection. ACS Sensors 2023, 8, 2263-70. (Supplementary Cover).
Chen, Y.-C.; Li, Y.; Yan, S. C.-C.; Hsu, C.-P.; Cheng, P.-L.*; Tu, H.-L.* DNA tension assays reveal that force-dependent integrin activation regulates neurite outgrowth in primary cortical neurons. Biomater. Adv. 2023, 150, 213431.
Gebreyesus, S. T.; Muneer, G.; Huang, I. C.-C.; Siyal, A.; Anand, M.; Chen, Y.-J.; Tu, H.-L.* Recent advances in microfluidics for single-cell functional proteomics. Lab Chip 2023, 23, 1726-51.
Montalbo, R.; Tu, H.-L.* Micropatterning of functional lipid membrane for quantitative bioanalysis. Biomicrofluidics 2023, 17, 031302.
Yin, M.-C.; Wei, P.-C.; Li, Y.; Hsu, T.; Chang, K.-C.; Lu, C.-P.; Tu, H.-L.; Wang, C.-M.* Structural variants and ultra-low detection ability for tryptamine in two polymorphs of a zincophosphate framework. Inorg. Chem. 2023, 62(44), 18150-56.
Herianto, S.; Chien, P.-J.; Ho, J.-A.; Tu, H.-L.* Liposome-based artificial cells: from gene expression to reconstitution of cellular functions and phenotypes. Biomater. Adv. 2022, 142, 213156.
Gebreyesus, S. T.; Siyal, A.; Kitata, R.; Chen, E. S.-W.; Enkhbayar, B.; Angata, T.; Lin, K.-I.; Chen, Y.-J.; Tu, H.-L.* Streamlined single-cell proteomics by an integrated microfluidic chip and data-independent acquisition mass spectrometry. Nat. Commun. 2022, 13, 37.
Chien, P.-J.; Shih, Y.-L.; Cheng, C.-T.; Tu, H.-L.* Chip-assisted formation of phase-separated liposomes for reconstituting spatial protein-lipid interactions. Lab Chip 2022, 22, 2540-48.
Kao, S.-H.; Liang, S.-Y.; Cheng, P.-L.; Tu, H.-L.* Surface viscosity-dependent neurite initiation in cortical neurons. Adv. Biol. 2022, 6, 2101325.
Ti, Y.-T.; Cheng, H.-C.; Li, Y.; Tu, H.-L.* Multiplexed patterning of hybrid lipid membrane and protein arrays for cell signaling study. Lab Chip 2021, 21, 2711-2720. (Back Cover).
Son, M.; Wang, A.; Tu, H.-L.; Metzig, M.; Patel, P.; Husain, K.; Lin, J.; Murugan, A.; Hoffmann, A.; Tay, S. NF-κB responds to absolute differences in cytokine concentrations. Sci. Signal. 2021, 14, eaaz4382.
Li, Y.; Xie, J.-F.; Chang, C.-C.; Wang, C.-M.*; Tu, H.-L.* Highly sensitive detection of mercury ion using zincophosphite framework nanoparticle-polyaniline composites. ACS Appl. Nano Mater. 2020, 3, 9724. (Supplementary Cover).
Zhang, C.; Tu, H.-L.; Jia, G.; Mukhtar, T.; Taylor, V.; Rzhtsky, A.; Tay, S. Ultra-Multiplexed Analysis of Single Cell Dynamics Reveals Logic Rules in Differentiation. Sci. Adv. 2019, 5, eaav7959.
Christensen, S.; Tu, H.-L.; Jun, J.; et al. One-way membrane trafficking of SOS in receptor-triggered Ras activation. Nat. Struct. Mol. Biol. 2016, 23, 838-846.
Iversen, L.; Tu, H.-L.; et al. Ras activation by SOS: Allosteric regulation by altered fluctuation dynamics. Science 2014, 345, 50-54.
Lin, W.-C.; Iversen, L.; Tu, H.-L.; et al. H-Ras forms dimers on membrane surfaces via a protein-protein interface. Proc. Natl. Acad. Sci. 2014, 111, 2996-3001.
Tu, H.-L.; Lin, Y.-S.; Lin, H.-Y.; Hung, Y.; Lo, L.-W.; Chen, Y.-F.; Mou, C.-Y. In-vitro studies of functionalized mesoporous silica nanoparticles for photodynamic therapy. Adv. Mater. 2009, 21, 172-177.
Tu, H.-L.; Tsai, F.-Y.; Mou, C.-Y.; et al. One step synthesis of ordered mesostructural organic/silica nanocomposite with tunable fluorescence surfactants. J. Mater. Chem. 2008, 18, 1771-1778.

Update： 2025-01-16

Gul Muneer, Sofani Gebreyesus, Ciao‐Syuan Chen, Tzu‐Tsung Lee, Fengchao Yu, Chih‐An Lin, M.‐S. Hsieh, A. I. Nesvizhskii, Chao‐Chi Ho, Sung‐Liang Yu, Hsiung‐Lin Tu*, and Yu‐Ju Chen* Mapping Nanoscale‐To‐Single‐Cell Phosphoproteomic Landscape by Chip‐DIA. Advanced Science 2024-10, 2402421, 2402421.
Subburaj Srinithi, Naveen Karuppusamy, S.M. Chen*, S. Balaji Natarajan, Balakumar Vellaichamy*, Ying Li, Hsiung-Lin Tu*, Chih-Min Wang*, and Yeh-Fang Duann* Ultra-sensitive zinc cobaltate assembled on N-rich carbon nitride electrochemical sensor for the detection of paraquat in food samples. Journal of the Taiwan Institute of Chemical Engineers 2024-10, 165, 105779.
Fei-Ting Hsu*, Ying-Tzu Chen, Yu-Cheng Chin, Li-Chan Chang, Shu-Chin Chiang, Li-Xing Yang, Hua-Shan Liu, Po-Fu Yueh, Hsiung-Lin Tu, Ruei-Yu He, Long-Bin Jeng, Woei-Cheang Shyu, Shang-Hsiu Hu, I-Tsang Chiang, Yu-Chang Liu, Yi-Chun Chiu, Guan-Chun Wu, Ching-Ching Yu, Wen-Pin Su*, and Chih-Chia Huang* Harnessing the Power of Sugar-Based Nanoparticles: A Drug-Free Approach to Enhance Immune Checkpoint Inhibition against Glioblastoma and Pancreatic Cancer. ACS Nano 2024-10, 18, 28764-28781.
Reynaldo C. Montalbo, Meng-Jie Wu and Hsiung-Lin Tu* One-step flow synthesis of size-controlled polymer nanogels in a fluorocarbon microfluidic chip. RSC Advances 2024-04, 14(16), 11258-11265.
Chieh-Teng Cheng, Jye-Chian Hsiao, Alexander Hoffmann and Hsiung-Lin Tu* TNFR1 mediates heterogeneity in single-cell NF-κB activation. iScience 2024-04, 27 (4), 109486.
Mu-Chien Yin, Pi-Chen Wei, Ying Li, Todd Hsu, Jia-Yi Jian, Kai-Chi Chang, Ching-Ping Lu, Hsiung-Lin Tu*, Chih-Min Wang* Structural Variants and Ultralow Detection Ability for Tryptamine in Two Polymorphs of a Zincophosphite Framework. Inorganic Chemistry 2023-11, 62(44), 18150-18156.
Narani Rakesh†, Hsiung‐Lin Tu†, Po‐Chun Chang, Sofani T. Gebreyesus, Che‐Jen Lin* Innovative real‐time flow sensor using detergent‐free complex emulsions with dual‐emissive semi‐perfluoroalkyl substituted <i>Α</i>‐cyanostilbene. Advanced Science 2023-11, 10(31), 2304108.
Ying-Chi Chen, Ying Li, Ching-Cher S. Yan, Chao-Ping Hsu, Pei-Lin Cheng* and Hsiung-Lin Tu* DNA tension assays reveal that force-dependent integrin activation regulates neurite outgrowth in primary cortical neurons. Biomaterials Advances 2023-07, 150, 213431.
Ying Li, Chiao-Chun Chang, Chu Wang, Wen-Ti Wu, Chih-Min Wang* and Hsiung-Lin Tu* Microfluidic biosensor decorated with an indium phosphate nanointerface for attomolar dopamine detection. ACS Sensors 2023-06, 8(6), 2263-2270.
Reynaldo Carlos K. Montalbo and Hsiung-Lin Tu* Micropatterning of functional lipid bilayer assays for quantitative bioanalysis (Invited Perspective). Biomicrofluidics 2023-05, 17(3), 031302.
Sofani Gebreyesus, Gul Muneer, Chih-Cheng Huang, Asad Siyal, Mihir Anand, Yu-Ju Chen and Hsiung-Lin Tu* Recent advances in microfluidics for single-cell functional proteomics. Lab on a Chip 2023-04, 23, 1726-1751.
Samuel Herianto, Po-Jen Chien, Ja-an Annie Ho and Hsiung-Lin Tu* Liposome-based artificial cells: From gene expression to reconstitution of cellular functions and phenotypes. Biomaterials Advances 2022-11, 142, 213156.
Chen Chen, Chien-Hsin Chu, Ying Chu, Ting-Ya Chang, Sheng-Wen Chen, Shu-Yang Liang, Yun-Chi Tsai, Bi-Chang Chen, Hsiung-Lin Tu and Pei-Lin Cheng* Neuronal paxillin and Drebrin mediate BDNF-induced force transduction and growth cone turning in a soft tissue-like environment. Cell Reports 2022-08, 40 (7), 111188.
Po-Jen Chien, Yi-Lun Shih, Chieh-Teng Cheng and Hsiung-Lin Tu* Chip assisted formation of phase-separated liposomes for reconstituting spatial protein–lipid interactions. Lab on a Chip 2022-06, 22, 2540-2548.
Shih-Han Kao, Shu-Yang Liang, Pei-Lin Cheng and Hsiung-Lin Tu* Surface viscosity-dependent neurite initiation in cortical neurons. Advanced Biology 2022-06, 6 (6), 2101325.
Sofani Gebreyesus, Asad Siyal, Reta Kitata, Eric Sheng-Wen Chen, Bayarmaa Enkhbayar, Takashi Angata, Kuo-I Lin, Yu-Ju Chen* and Hsiung-Lin Tu* Streamlined single-cell proteomics by an integrated microfluidic chip and data-independent acquisition mass spectrometry. Nature Communications 2022-01, 13, 37.
Asad Siyal, Eric Sheng-Wen Chen, Hsin-Ju Chan, Reta Birhanu Kitata, Jhih-Ci Yang, Hsiung-Lin Tu and Yu-Ju Chen* Sample Size-Comparable Spectral Library Enhances Data-Independent Acquisition-Based Proteome Coverage of Low-Input Cells. Analytical Chemistry 2021-12, 93 (51), 17003-17011.
Chu Wang , Jia-Rong Li, Ying Li, Hsiung-Lin Tu, Der-Lii M Tzou and Chih-Min Wang* Stable Crystalline Organic-Inorganic Hybrid Indium Phosphate with Dye Removal and Ractopamine Detection Applications. Inorganic Chemistry 2021-07, 60 (15), 11655-11660.
Yu-Ting Ti, Hsiao-Chi Cheng, Ying Li and Hsiung-Lin Tu* Multiplexed patterning of hybrid lipid membrane and protein arrays for cell signaling study. Lab on a Chip 2021-07, 21, 2711-2720.
Da-Wei Lin, Yang Liu, Yue-Qi Lee, Po-Jiun Yang, Chia-Tse Ho, Jui-Chung Hong, Jye-Chian Hsiao, Der-Chien Liao, An-Jou Liang, Tzu-Chiao Hung, Yu-Chuan Chen, Hsiung-Lin Tu, Chao-Ping Hsu and Hsiao-Chun Huang* Construction of intracellular asymmetry and asymmetric division in Escherichia coli. Nature Communications 2021-02, 12, 888.
Minjun Son, Andrew Wang, Hsiung-Lin Tu, Marie Metzig, Parthiv Patel, Kabir Husain, Jing Lin, Arvind Murugan, Alexander Hoffmann and Savaş Tay* NF-κB responds to absolute differences in cytokine concentrations. Science Signaling 2021-01, 14 (666), eaaz4382.
Ying Li, Ji-Fang Xie, Chiao-Chun Chang, Chih-Min Wang * and Hsiung-Lin Tu* Highly Sensitive Detection of Mercury Ions Using Zincophosphite Framework Nanoparticle-Polyaniline Composites. ACS Applied Nano Materials 2020-09, 3 (10), 9724-9730.
Jui Hsieh, Ju-Ying Chen, Han-Ying Li, Hsin-Kuan Liu, Hsiung-Lin Tu and Chih-Min Wang* A thio-functionalized zinc phosphite with a large-channel framework and enhanced removal ability of mercury ion from aqueous solutions. Dalton Transactions 2020-06, 49 (32), 11085-11089.
Udesh Dhawan, Wei‐Li Wang, Bhaskarchand Gautam, Jayakrishnan Janardhanan, Po‐Chiang Hsiao, Hsiung‐Lin Tu and Hsiao‐Hua Yu* Mechanotactic Activation of TGF‐β by PEDOT Artificial Microenvironments Triggers Epithelial to Mesenchymal Transition. Advanced Biosystems 2020-01, 4 (2), 1900165.
Lin Jing, Jordi Christian, Son Minjun, Van Phan Hoang, Drayman Nir, Abasiyanik Mustafa Fatih, Vistain Luke, Tu Hsiung-Lin, Tay Savaş Ultra-sensitive digital quantification of proteins and mRNA in single cells. Nature Communications 2019-08, 10(1), 3544.
Zhang Ce, Tu Hsiung-Lin, Jia Gengjie, Mukhtar Tanzila, Taylor Verdon, Rzhetsky Andrey, Tay Savaş Ultra-multiplexed analysis of single-cell dynamics reveals logic rules in differentiation. Science Advances 2019-04, 5(4), eaav7959.
Sune M Christensen, Hsiung-Lin Tu, Jesse E Jun, Steven Alvarez, Meredith G Triplet, Jeffrey S Iwig, Kamlesh K Yadav, Dafna Bar-Sagi, Jeroen P Roose, Jay T Groves One-way Membrane Trafficking of SOS in Receptor-Triggered Ras Activation. NATURE STRUCTURAL & MOLECULAR BIOLOGY 2016, 23, 838-846.
William Y. C. Huang, Qingrong Yan, Wan-Chen Lin, Jean K. Chung, Scott D. Hansen, Sune M. Christensen, Hsiung-Lin Tu, John Kuriyan, Jay T. Groves Phosphotyrosine-mediated LAT Assembly on Membranes Drives Kinetic Bifurcation in Recruitment Dynamics of The Ras Activator SOS. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2016, 113, 8218-8223.
Sune M. Christensen, Meredith G. Triplet, Christopher Rhodes, Jeffrey S. Iwig, Hsiung-Lin Tu, Dimitrios Stamou, Jay T. Groves Monitoring The Waiting Time Sequence of Single Ras GTPase Activation Events Using Liposome Functionalized Zero Mode Waveguides. NANO LETTERS 2016, 16, 2890-2895.
Lars Iversen, Hsiung-Lin Tu, Wan-Chen Lin, Sune M. Christensen, Steven M. Abel, Jeff Iwig, Hung-Jen Wu, Jodi Gureasko, Christopher Rhodes, Rebecca S. Petit, Scott D. Hansen, Peter Thill, Cheng-Han Yu, Dimitrios Stamou, Arup K. Chakraborty, John Kuriyan, Ras Activation by SOS: Allosteric Regulation by Altered Fluctuation Dynamics. SCIENCE 2014, 345, 50-54.
Wan-Chen Lin, Lars Iversen, Hsiung-Lin Tu, Christopher Rhodes, Sune M. Christensen, Jeffrey S. Iwig, Scott D. Hansen, William Y. C. Huang, Jay T. Groves H-Ras Forms Dimers on Membrane Surfaces via A Protein-Protein Interface. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2014, 111, 2996-3001.
T. Lohmüller, L. Iversen, M. Schmidt, C. Rhodes, Hsiung-Lin Tu, W.-C. Lin, J. T. Groves Single Molecule Tracking on Supported Membranes with Arrays of Optical Nanoantennas. NANO LETTERS 2012, 12, 1717-1721.
Hsiung-Lin Tu, Yu-Shen Lin, Hsia-Yu Lin, Yann Hung, Leu-Wei Lo, Yang-Fang Chen, Chung-Yuan Mou In-vitro Studies of Functionalized Mesoporous Silica Nanoparticles for Photodynamic Therapy. ADVANCED MATERIALS 2009, 21, 172-177.
Hsiung-Lin Tu, Fu-Yu Tsai, C.-Y. Mou One Step Synthesis of Ordered Mesostructural Organic/silica Nanocomposite with Tunable Fluorescence Surfactants. JOURNAL OF MATERIALS CHEMISTRY 2008, 18, 1771-1778.
Fu-Yu Tsai, Hsiung-Lin Tu, Chung-Yuan Mou. Nonionic Fluorescent Oligomeric Surfactant for Ordered Mesoporous Silica Structure. JOURNAL OF MATERIALS CHEMISTRY 2006, 16, 348-350.