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黃人則 (Joseph Jen-Tse Huang)

化學生物學、生物物理化學、奈米科學

黃人則 (Joseph Jen-Tse Huang)

    886-2-55728652
    886-2-27831237
    jthuanggate.sinica.edu.tw
researcherid:E-4918-2015
ORCID id:0000-0003-4847-7126
研究員(109年迄今)；副研究員(102至109年)；助研究員(96至102年)；神經科學研究計畫成員(NPAS)(97年迄今)；國立清華大學化學學士(81至85年)；國立清華大學化學碩士(85至87年)；國立台灣大學化學博士(89至93年)；中央研究院化學研究所博士後研究(93至94年)；美國威斯康辛大學化學系博士後研究(94至96年)；第一屆 國科會菁英計畫(94年至96年)；中國化學會傑出青年化學獎章(101年)；科技部優秀年輕學者研究計畫(106)；第三屆漸凍人研究獎(107)；中研院前瞻計畫(108)；
研究領域

黃人則老師研究團隊目前的主要目標有 (一) 闡明蛋白質聚集體在神經退化性疾病之致病機轉中扮演的角色，並發展新穎胜肽藥物治療方針；以及 (二) 研究蛋白質共轉譯過程中，其摺疊、導向及運輸可能機制。

在許多神經退化性疾病的細胞中，都發現有不正常蛋白質聚集體的存在，它們共同的特質包含纖維狀型態、富含 beta 摺版的二級結構，但這些蛋白質聚集體的分子形成機制、在細胞層次上如何影響疾病進程，仍待進一步探究。TDP-43 蛋白質被認為是肌萎縮性側索硬化症 (ALS；漸凍人) 和腦前額顳葉退化症 (FTLD) 的致病因子，黃老師團隊過去的研究顯示含 TDP-43 蛋白質中 C 端特定片段序列之胜肽，能夠透過晶種效應加速 TDP-43 蛋白質聚集化並形成纖維狀聚集體。

目前，黃老師團隊透過合成具「光控制性質」的胜肽，配合即時偵測細胞影像之技術，發展一套化學生物學工具，以探討細胞中 TDP-43 蛋白質及其他神經退化性疾病相關致病蛋白 (如杭丁頓氏舞蹈症中之 Huntingtin) 的詳細特性與行為。光控制胜肽在適當波長的光照射之下將斷裂，並開始自組裝聚集化，或誘導其他細胞內蛋白質聚集化。透過準確調控照光之時間點及空間範圍，我們能夠更仔細研究這些蛋白質聚集體以及它們在相關疾病中扮演的角色。目前具體研究方向包含：

    1. 光控制胜肽之設計與合成
    2. 設計新穎螢光染劑
    3. 利用神經細胞株/人類誘導幹細胞研究特定蛋白質在相關疾病中的角色
    4. 結合奈米科學，將胜肽修飾於奈米粒子表面，研究並開發高效率胜肽藥物輸送方式以及生物感測器。

另一方面，疾病中蛋白質聚集體的形成與蛋白質錯誤摺疊亦有關連。目前已知，活體細胞中蛋白質的合成及其正確摺疊受到各種不同類型的伴隨蛋白 (chaperon) 所調控，此過程的錯誤將會導致蛋白質不正常堆疊而進而疾病。因此，黃老師研究團隊也針對蛋白質轉譯過程中，核醣體和伴隨蛋白之間的交互作用關係進行研究。

實驗室成功建立之化學生物學平台，能夠製備螢光標記胺基酸-tRNA 並將之用於無細胞系統進行蛋白質轉譯，表達在任意特定位置具有螢光標記之目標蛋白質。利用該技術，目前研究已經指出伴隨蛋白 Trigger Factor 與 DnaK 會對新生蛋白質-核糖體複合物的動態結構有不同之影響，也正進一步探討兩者對於蛋白質摺疊機制之作用。這些各式伴隨蛋白之研究，將啟發針對與蛋白質錯誤摺疊相關疾病之新式藥物設計。

ACS Appl. Mater. Interfaces 2021

Adv. Sci. 2020

ACS Nano 2017

chem. Commun. 2015

J. Am. Chem. Soc. 2010

重要獎項及殊榮
  • "The 2019 Career Development Award (中研院前瞻計畫)"  (2019)
  • "Award received from the Taiwan Motor Neuron Disease Association(第三屆漸凍人研究獎)"  (2018)
  • "Ministry of Science and Technology Research Project for Excellent Young Scholars(科技部優秀年輕學者研究計畫)"  (2017)
  • 2012年中國化學會傑出青年化學獎章(2012 Excellent Young Scholar Award)  (2012)
  • 菁英專案擴增留學計畫獎助(Taiwan Merit Scholarship)  (2005)
  • 台灣大學理學院院長獎  (2004)
  • 第八屆生物物理年會壁報論文比賽第一名  (2002)
代表作
    JOURNAL ARTICLES:
    1. Wahyuningtyas, D.; Chen, W.-H.; He, R.-Y.; Huang, Y.-A.; Tsao, C.-K.; He,Y.-J.; Yu,C.-Y.; Lu, P.-C.; Chen,Y.-C.; Wang, S.-H.; Ng, K.-C.; Chen, P.-W.; Wei, P.-K.; Shie, J.-J.; Kuo, C.-H.; Sun,Y.-H.; Huang, J. J.-T.*  Polyglutamine-Specific Gold Nanoparticle Complex Alleviates Mutant Huntingtin-Induced Toxicity. ACS Appl. Mater. Interfaces 2021, 13, 60894−60906.
    2. Chien, H. -M.; He, R. -Y.; Lee, C. -C.; Huang, Y. -A.; Hung, I. -J.; Hou, K. -T.; Hsiao, J. -C.; Lu, P. -C.; Agnihotri, D.; Hwang, E.; Huang, J. J.-T.* Nanoscopic investigation of C9orf72 poly-GA oligomers on nuclear membrane disruption by photoinducible platform. Commun. Chem. 2021, 4, 111.
    3. He, R.-Y.; Lai, X.-M.; Sun, C. -S; Kung, T.-S.; Hong, J.-Y.; Jheng, Y.-S.; Liao, W. -N.; Chen, J. -K.; Liao, Y. -F.; Tu, P. -H.; Huang, J. J. -T. Nanoscopic insights of amphiphilic peptide against oligomer assembly process to treat Huntington’s disease. Adv. Sci. 2020, 7, 1901165.
    4. Wahyuningtyas, D.; Chen, W.-H.; Huang, C.-H.; He, Y.-J.; Huang, J.J-T. Biocompatible Inhibitor Based on Chitosan and Amphiphilic Peptide against Mutant Huntingtin Toxicity. ChemBioChem 2019, 20, 2133-2140. (Cover Article)
    5. Lee, C.-C.; He, R.-Y.; Huang, J.J-T. Learning from the TDP-43 amyloidogenic sequences in neurodegenerative diseases. Amyloid 2019, 26, 154-155.
    6. He, R.-Y.; Chao, S.-H.; Tsai, Y.-J.; Lee, C.-C.; Yu, C.-Y.; Gao, H.-D.; Huang, Y.-A.; Hwang, E.; Lee, H.-M.; Huang, J. J-T. Photo-Controllable Probe Spatiotemporally Induces Neurotoxic Fibrillar Aggregates and Impairs Nucleocytoplasmic Trafficking. ACS Nano 2017, 11 (7), 6795-6807.
    7. Du, Y.-P.; Chang, H.-H.; Yang, S.-Y.; Huang, S.-J.; Tsai, Y.-J.; Huang, J..J.-T.; Chan, J.-C. Study of Binding Interaction between Pif80 Protein Fragment and Aragonite. Sci. Rep. 2016, 6, 30883.
    8. Chen, C-H.; Khan, A.; Huang, J. J.-T.; Ulmschneider, M.-B. Mechanisms of membrane-pore formation by amyloidogenic peptides in ALS.Chem. Eur. J. 2016, 22, 9958-9961.
    9. He, R.-Y.; Huang, Y.-C.; Chiang, C.-W.; Tsai, Y.-J.; Ye, T.-J.; Gao, H.-D.; Wu, C.-Y.; Lee, Y.-M.; Huang, J. J.-T. Characterization and real-time imaging of the FTLD-related protein aggregation induced by amyloidogenic peptides. Chem. Commun. 2015, 51, 8652.
    10. Sun, C.-S. ; Lee, C.-C.; Li, Y-N; Yang S. Y.-C.; Lin, C.-H.; Chang, Y.-C.; He, R.-Y.; Liu, P.-F.; Wang ,C.-H.; Chen, W.; Chern, Y.; Huang J. J.-T. Conformational switch of polyglutamine-expanded huntingtin into benign aggregates leads to neuroprotective effect. Sci. Rep. 2015, 5, 14992. (Recommended in F1000Prime by F1000 faculty member)
    11. Huang C.-C.; Bose J.-K.; Majumder P.; Lee K.-H., Huang, J. J.-T.; Huang J. K.; Shen J. C.-K. Metabolism and mis-metabolism of the neuropathological signature protein TDP-43. J. Cell Sci. 2014, 127(Pt 14), 3024..
    12. Liu, G. C.-H.; Chen, B. P.-W.; Ye, N. T.-J.; Wang, C.-H.; Chen, W.; Lee, H.-M.; Chan, S. I., Huang, J. J.-T. Delineating the membrane-disrupting and seeding properties of the TDP-43 amyloidogenic core. Chem. Commun. 2013, 49, 11212.
      (Highlighted in Significant Research Achievements of Academia Sinica 2013)
    13. Huang, J. J.-T.; Larsen, R.-W.; Chan, S.-I. The Interplay of Turn Formation and Hydrophobic Interactions on the Early Kinetic Events in Protein Folding. Chem. Comm. 2012, (Feature Article), 48, 487.
    14. Chen, K.-H.; Lin, Y.-Y.; Xie, Z.-J.; Tu, P.-H.; Chen, P.-Y.; Liao, T.-Y.; Huang, J. J.-T. Induction of Amyloid Fibrils by the C-terminal Fragments of TDP-43 in Amyotrophic Lateral Sclerosis. J. Am. Chem. Soc. 2010, 132, 1186.
      (Highlighted in Significant Research Achievements of Academia Sinica 2010)
      (Selected in 2010 Science and Technology Yearbook of Republic of China)
    15. Kuo, N. N.-W.; Huang, J. J.-T.; Miksovska, J.; Chen, P.-Y.; Larsen, R.; Chan, S. I. Effects of turn stability on the kinetics of refolding of a hairpin in b-sheet. J. Am. Chem. Soc. 2005, 127, 16945.
    16. Chen, R. P. Y.; Huang, J. J.-T.; Chen, H. L.; Jan, H.; Velusamy, M.; Lee, C. T.; Fann, W. S.; Larsen, R. W.; Chan, S. I. Measuring the refolding of beta-sheets with different turn sequences on a nanosecond time scale. Proc. Natl. Acad. Sci. U. S. A. 2004, 101, 7305.
      BOOK CHAPTER:
    1. Chan, S. I.; Huang, J. J.-T.; Larsen, R. W.; Rock, R. S.; Hansen, K. C. Early kinetic events in protein folding: The development and applications of caged peptides in dynamic studies in biology. phototriggers, photoswitches, and caged biomolecules; Goeldner, M. and Givens. R. (eds), Wiley-VCH GmbH & Co. Germany, 2005, pp479.

    Update： 2022-01-05

期刊論文
  1. Chen, B. P.-W., He, R.-Y., Chien, H.-M., Lee, C.-C., Hsu, C.-P., Chan, J .C.-C., Huang, J. J.-T.* Superresolution Imaging of Photochromic Acylhydrazone Moieties on Amyloid Nanofibrils: Implications for Photoswitchable Probes.. ACS APPLIED NANO MATERIALS 2022-01, 5(2), 1734-1739.
  2. Wahyuningtyas, D., Chen, W.-H., He, R.-Y., Huang, Y.-A., Tsao, C.-K., He, Y.-J., Yu, C.-Y., Lu, P.-C., Chen, Y.-C., Wang, S.-H., Ng, K.-C., Chen, P.-W., Wei, P.-K., Shie, J.-J., Kuo, C.-H., Sun, Y.-H., Huang, J. J.-T.* Polyglutamine-Specific Gold Nanoparticle Complex Alleviates Mutant Huntingtin-Induced Toxicity. ACS APPLIED MATERIALS & INTERFACES 2021-12, 13, 60894-60906.
  3. Chien, H.-M., Lee, C.-C., Huang, J. J.-T.* The Different Faces of the TDP-43 Low-Complexity Domain: The Formation of Liquid Droplets and Amyloid Fibrils. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 2021-07, 22(15), 8213.
  4. Chien, H.-M., He, R.-Y., Lee, C.-C., Huang, Y.-A., Hung, I.-J., Hou, K.-T., Hsiao, J.-C., Lu, P.-C., Agnihotri, D., Hwang, E., Huang, J. J.-T.* Nanoscopic investigation of C9orf72 poly-GA oligomers on nuclear membrane disruption by photoinducible platform. Communications Chemistry 2021-07, 4(111), 1-13.
  5. He, R. -Y.*, Lai, X.-M., Sun, C. -S, Kung, T.-S., Hong, J.-Y., Jheng, Y.-S., Liao, W. -N., Chen, J. -K., Liao, Y. -F., Tu, P. -H.*, Huang, J. J.-T.* Nanoscopic insights of amphiphilic peptide against oligomer assembly process to treat Huntington’s disease. ADVANCED SCIENCE 2019-12, 1901165.
  6. Lee, C.-C.,He, R.-Y., Huang, J.J-T.* Learning from the TDP-43 amyloidogenic sequences in neurodegenerative diseases. AMYLOID-JOURNAL OF PROTEIN FOLDING DISORDERS 2019-07-25, 26, 154-155..
  7. Wahyuningtyas, D., Chen, W.-H., Huang, C.-H., He, Y.-J., Huang, J.J-T.* Biocompatible Inhibitor Based on Chitosan and Amphiphilic Peptide against Mutant Huntingtin Toxicity. CHEMBIOCHEM 2019-06, 20(16), 2133-2140..
  8. He, R.-Y., Chao, S.-H., Tsai, Y.-J., Lee, C.-C., Yu, C.-Y., Gao, H.-D., Huang, Y.-A., Hwang, E., Lee, H.-M., Huang, J.J-T.* Photo-controllable probe spatiotemporally induces neurotoxic fibrillar aggregates and impairs nucleocytoplasmic trafficking. ACS Nano 2017-08, 11 (7), 6795-6807.
  9. Koubek, J., Chang, Y.-C., Yang, S.Y.-C., Huang, J.J-T.*  Trigger factor-induced nascent chain dynamics changes suggest two different chaperone-nascent chain interactions during translation. JOURNAL OF MOLECULAR BIOLOGY 2017-07, 429(11),1733-1745.
  10. Chen, C.-H. , Khan, A. , Huang, J. J.-T. , Ulmschneider, M.-B.* Mechanisms of membrane-pore formation by amyloidogenic peptides in ALS. CHEMISTRY-A EUROPEAN JOURNAL 2016, 22, 9958-9961.
  11. Du, Y.-P., Chang,H.-H., Yang, S.-Y., Huang, S.-J., Tsai, Y.-J., Huang,J.J.-T.,Chan,J.C.-C. Study of Binding Interaction between Pif80 Protein Fragment and Aragonite. SCIENTIFIC REPORTS 2016, 6,30883.
  12. Sun, C.-S. , Lee, C.-C., Li, Y-N, Yang S. Y.-C., Lin, C.-H., Chang, Y.-C., He, R.-Y. , Liu, P.-F., Wang ,C.-H., Chen, W. , Chern, Y., Huang, J. J.-T.*  Conformational switch of polyglutamine-expanded huntingtin into benign aggregates leads to neuroprotective effect.. Scientific Reports 2015, 5,14992.
  13. Koubek, J., Chen, Y.-R., Cheng, R.-P.,Huang, J.J.-T.* Nonorthogonal tRNA(cys, Amber) for protein and nascent chain labeling. RNA 2015, 21,1672-1682.
  14. He, R.-Y., Huang, Y.-C., Chiang, C.-W., Tsai, Y.-J., Ye, T.-J., Gao, H.-D., Wu, C.-Y., Lee, Y.-M., Huang, J.J.-T.*  Characterization and real-time imaging of the FTLD-related protein aggregation induced by amyloidogenic peptides. CHEMICAL COMMUNICATIONS 2015, 51, 8652-8655.
  15. Sun, C.-S., Wang, C.-Y., Chen, B.-P., He, R.-Y., Liu, G. C.-H., Wang, C.-H., Chen, W., Chern. Y., Huang, J. J.-T.* The Influence of Pathological Mutations and Proline Substitutions in TDP-43 Glycine-Rich Peptides on Its Amyloid Properties and Cellular Toxicity. PloS one 2014, 9(8), e103644.
  16. Huang, C.-C., Bose, J.-K., Majumder, P., Lee, K.-H., Huang, J. J.-T., Huang, J.-K., Shen, C.-K.*. Metabolism and mis-metabolism of the neuropathological signature protein TDP-43. Journal of cell science 2014, 127(Pt 14), 3024-38.
  17. Liu, G. C.-H., Chen, B. P.-W, Ye, N. T.-J., Wang, C.-H., Chen, W., Lee, H.-M., Chan, S.-I., Huang, J.J.-T.* Delineating the membrane-disrupting and seeding properties of the TDP-43 amyloidogenic core. CHEMICAL COMMUNICATIONS 2013-10, 49(95), 11212-4.
  18. Koubek, J., Lin, K.-F., Chen, Y.-R., Cheng, R.-P., Huang, J. J.-T.* Strong anion exchange fast performance liquid chromatography as a versatile tool for preparation and purification of RNA produced by in vitro transcription. RNA 2013-06, 19,1449-1459.
  19. Huang, Y.-C. Lin, K.-F., He, R.-Y., Tu, P.-H. Koubek, J., Hsu,Y.-C., Huang, J. J.-T.*  Inhibition of TDP-43 aggregation by nucleic acid binding. PLoS One 2013-05, 8(5), e64002.
  20. Huang, J. J.-T., Larsen, R.-W., Chan, S.-I.* The Interplay of Turn Formation and Hydrophobic Interactions on the Early Kinetic Events in Protein Folding. CHEMICAL COMMUNICATIONS 2012, 48, 487-497.
  21. Lin, K.-F., Sun, C.-S., Huang, Y.-C., Chan, S.-I., Koubek, J., Wu, T.-H., Huang, J. J.-T.* Cotranslational Protein Folding within the Ribosome Tunnel Influences Trigger-Factor Recruitment. BIOPHYSICAL JOURNAL 2012, 102, 2818-2827.
  22. Chang, C.-K., Wu, T.-H., Wu, C.-Y., Chiang, M.-H., Toh, E.-K., Hsu, Y.-C., Lin, K.-F., Liao, Y.-H., Huang, T.-H.*, Huang, J. J.-T.* The N-terminus of TDP-43 Promotes Its Oligomerization and Enhances DNA Binding Affinity.. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 2012, 425(2), 219-224.
  23. Allan K.-H. Chen, Ryan Y.-Y. Lin, Eva Z.-J. Hsieh, Pang-Hsien Tu, Rita P.-Y. Chen,Tai-Yan Liao, Wenlung Chen, Chih-Hsien Wang, and Joseph J.-T. Huang* Induction of Amyloid Fibrils by the C-Terminal Fragments of TDP-43 in Amyotrophic Lateral Sclerosis. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2010, 132,1186-1187.
  24. Jih Ru Hwu,*Joseph Jen-Tse Huang,Fu-Yuan Tsai,Shwu-Chen Tsay,Ming-Hua Hsu,Kuo Chu Hwang,Jia-Cherng Horng,Ja-an Annie Ho,Chun-Cheng Lin Photochemical Activities of N-Nitroso Carboxamides and Sulfoximides as well as Their Application to DNA Cleavage. CHEMISTRY-A EUROPEAN JOURNAL 2009, 15, 8742-8750.
  25. Huang, J. J.-T.*, Jhan, J.-W. Ultra-fast and Cotranslational Protein Folding. Natural Sciences Newsletter 2008, 20,50-53.
  26. Kirchdoerfer RN, Huang JJ, Isola MK, Cavagnero S Fluorescence-based analysis of aminoacyl- and peptidyl-tRNA by low-pH sodium dodecyl sulfate-polyacrylamide gel electrophoresis. ANALYTICAL BIOCHEMISTRY 2007, 364(1), 92-4.
  27. Kuo NN, Huang JJ, Miksovska J, Chen RP, Larsen RW, Chan SI Effects of turn stability on the kinetics of refolding of a hairpin in a beta-sheet. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2005, 127(48), 16945-54.
  28. Chen RP, Huang JJ, Chen HL, Jan H, Velusamy M, Lee CT, Fann W, Larsen RW, Chan SI Measuring the refolding of beta-sheets with different turn sequences on a nanosecond time scale. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2004, 101(19), 7305-10.
專書、論文集
專書(論文集)之一章
  1. Chan, S. I.*, Huang, J. J.-T., Larsen, R. W., Rock, R. S., Hansen, K. C., 2005, “Early kinetic events in protein folding: The development and applications of caged peptides.”, editor(s): Goeldner, M., Givens. R., Dynamic Studies in Biology: Phototriggers, Photoswitches and Caged Biomolecules, pp. 479-494, Germany: Wiley-VCH GmbH & Co..
學術會議(研討會)論文
  1. Jen-Tse Huang*, 2018, “Identify amyloidogenic peptides in TDP-43 and create photocontrollable probes for neurodegenerative disease”, paper presented at The 10th International Peptide Symposium, Japan: The Liaison Committee of International Peptide Societies,, 2018-12-03 ~ 2018-12-07.
  2. Jen-Tse Huang*, 2018, “Learning from the Amyloidogenic Peptides in Amyotrophic Lateral Sclerosis ”, paper presented at The XVIth International Symposium on Amyloidosis, Kumamoto, Japan: ISA2018 program, 2018-05-26 ~ 2018-05-29.
  3. Jen-Tse Huang*, 2017, “Photo-Controllable Probe Spatiotemporally Induces Neurotoxic Aggregates in Neurodegenerative Disease”, paper presented at BIT’s 7th Annual World Congress of Nano Science & Technology-2017, Fukuoka, Japan: World High Technology Society (WHTS), 2017-10-24 ~ 2017-10-26.
  4. Jen-Tse Huang*, 2016, “Amyloidogenic Peptides and Proteins inNeurodegenerative Diseases”, paper presented at 2016 ITbM-IoC Joint Workshop on Biomolecules and Materials, Nagoya University, Nagoya, Japan: Institute of Transformative Bio-Molecules Nagoya University, 2016-11-15 ~ 2016-11-17.
  5. Jen-Tse Huang*, 2016, “Learning from the Amyloidogenic Peptides in the Neurodegenerative Diseases”, paper presented at KAUST-NTU-AS Workshop for Enhancing collaborations on Research and Education in Chemical and Materials Sciences and Engineering, King Abdullah University of Science and Technology, Thuwal/Saudi Arabia: King Abdullah University of Science and Technology, KAUST, 2016-04-01 ~ 2016-04-08.
  6. Jen-Tse Huang*, 2015, “ALS and FTLD-related protein aggregation induced by amyloidogenic peptides”, paper presented at PACIFICHEM 2015, Honolulu, Hawaii, USA: The International Chemical Congress of Pacific Basin Societies, 2015-12-15 ~ 2015-12-20.
  7. VJ,Joseph J.-T. Huang*, 2015, “Early recognition by SecA targets secretory protein near the ribosome exit tunnel”, paper presented at Protein synthesis and translational control, Germany: The European Molecular Biology Laboratory, 2015-09-09 ~ 2015-09-13.
  8. Koubek J,Joseph J.-T. Huang*, 2015, “The local and global effects of DnaK and Trigger Factor on ribosome-bound nascent chain dynamics”, paper presented at Molecular chaperones: From molecules to cells and misfolding diseases, Greece: EMBO Conference, 2015-05-08 ~ 2015-05-13.
  9. Jen-Tse Huang*, 2015, “From pathological to therapeutic peptides in neurodegenerative diseases”, paper presented at 分子科學前沿海峽兩岸交流會, 北京中國科學院化學研究所: 中國科學院化學研究所, 2015-04-24 ~ 2015-04-27.
  10. He Ruei-Yu,Jen-Tse Huang*, 2014, “Synthetic Gluntamine/Asparagine-rich polypeptides from TDP-43 exhibit amyloid properties and induce prion-like aggregation in amyotrophic lateral scelerosis”, paper presented at The 2nd Taiwan-Tohoku University Neuroscience Workshop for Young Scientists, Japan: Taiwan-Tohoku University Neuroscience Workshop for Young Scientists, 2014-12-07 ~ 2014-12-10.
  11. Joseph Jen-Tse Huang, 2014, “The amyloid and prion-like properties of the TDP-43 peptides in Amyotrophic Lateal Sclerosis”, paper presented at The 2nd Taiwan-Tohoku University Neuroscience Workshop for Young Scientists, Japan: The 2nd Taiwan-Tohoku University Neuroscience Workshop for Young Scientists, 2014-12-07 ~ 2014-12-10.
  12. Koubek J,Joseph J.-T. Huang*, 2014, “Development of cysteine-specific tRNA and tRNA synthetase for site-specific protein fluorescence labeling in co-translational protein folding studies”, paper presented at 25th tRNA Conference, Greece: Society of Biochemistry and Molecular Biology, the University of Patras, 2014-09-21 ~ 2014-09-25.
  13. Gerard, Chun-Hao Liu, Bryan, Po-Wen Chen, Nancy, Ting-Juan Ye, Chih-Hsien Wang, Wenlung Chen, Hsien-Ming Lee, Sunney I. Chan, Joseph, Jen-Tse Huang*, 2014, “Delineating the membrane-disrupting and seeding properties of the TDP-43 amyloidogenic core”, paper presented at The 19th Annual Conference of the Biophysical Society of ROC, National Chen Kung University, Tainan, Taiwan: Biophysical Society of R.O.C.Institute of Bioinformatics and Biosignal Transduction, CBB, National Chen-Kung University, 2014-05-07 ~ 2014-05-10.
  14. Chia-Sui Sun, Yijuang Chern, Joseph J.-T. Huang*, 2014, “The modulation of FKBP12 on the mutant Huntintin-induced neurotoxicity and aggregation”, paper presented at The 19th Annual Conference of the Biophysical Society of ROC, National Chen Kung University, Tainan, Taiwan: Biophysical Society of R.O.C.Institute of Bioinformatics and Biosignal Transduction, CBB, National Chen-Kung University, 2014-05-07 ~ 2014-05-10.
  15. Huang, J. J.-T.*, 2013, “From pathological to therapeutic peptides”, paper presented at The Hebrew University of Jerusalem, Israel - Academia Sinica Bilateral Workshop on Nanoscience and Nanotechnology, The Auditorium, 1F, Institute of Physics, Academia Sinica: Academia Sinica Research Program on Nanoscience and Nanotechnology, 2013-11-15.
  16. Huang, J. J.-T.*, 2013, “The Impact of co-Translational Protein Folding within the Ribosome Tunnel on Chaperone Recruiitment”, paper presented at Technologies for Medical Diagnosis and Therapy Symposium, B1 Auditorium, Inst. of Biomedical Sciences, Academia Sinica: Institute of Biomedical Sciences Academia Sinica, 2013-10-21 ~ 2013-10-22.
  17. Huang, J. J.-T.*, 2013, “Complementary paradigm and insight towardmodulating TDP-43 aggregation via nucleic acid binding”, paper presented at Institute for Protein Research Seminar 2013, Osaka, Japan: Institute for Protein Research, 2013-06-19 ~ 2013-06-20.
  18. Wu, T.-H., Chang, C.-K., Wu, C.-Y. Hsu, Y.-C., Huang, T.-H., Huang, J. J.-T.*, 2013, “C-terminal aggregation-prone domain of neurodegenerative TDP-43 is required for altering ssDNA/RNA structure”, paper presented at The 18th Biophysics Conference, Institute of BioMedical Sciences, Academia Sinica: Biophysical Society of R.O.C、IBMS, Academia Sinica, 2013-06-27 ~ 2013-06-29.
  19. Hsu, Y.-C., Lin, K.-F., Huang, J. J.-T*, 2013, “Characterization of the thermal induced fiber from TDP-43 and its association with single-stranded DNA”, 83 pages, paper presented at The 18th Biophysics Conference, Institute of BioMedical Sciences, Academia Sinica: Biophysical Society of R.O.C、IBMS, Academia Sinica, 2013-06-27 ~ 2013-06-29.
  20. Huang, Y.-C., Lin, K.-F., He, R.-Y., Tu, P.-H. Koubek, J., Hsu,Y.-C., Huang, J. J.-T.* , 2013, “Inhibition of TDP-43 aggregation by nucleic acid binding”, 77 pages, paper presented at The 18th Biophysics Conference, Institute of BioMedical Sciences, Academia Sinica: Biophysical Society of R.O.C、IBMS, Academia Sinica, 2013-06-27 ~ 2013-06-29.
  21. Chang, C.-K., Chiang, M.-H., Wu, T.-H., Toh, E. K.-W., Huang, J. J.-T., Huang, T.-H.*, 2013, “Directional binding of nucleic acid to TAR DNA-binding protein (TDP-43) RRM1 domain provides insight into splicing regulation”, 69 pages, paper presented at The 18th Biophysics Conference, Institute of BioMedical Sciences, Academia Sinica: Biophysical Society of R.O.C、IBMS, Academia Sinica, 2013-06-27 ~ 2013-06-29.
  22. Vijayasarathy.K, Hsu Yin-Chih, Jiri Koubek, Joseph J.-T. Huang*, 2013, “Structure characterization of ATPase-chaperone-preprotein recognition in the secretory pathway”, 84 pages, paper presented at The 18th Biophysics Conference, Institute of BioMedical Sciences, Academia Sinica: Biophysical Society of R.O.C、IBMS, Academia Sinica, 2013-06-27 ~ 2013-06-29.
  23. Li, Y.-N., Sun, C.-S., Wu. C.-Y., Huang. J. J.-T.*, 2013, “Modulate polyglutamin protein aggregation by Trigger factor and FKBP12”, 79 pages, paper presented at The 18th Biophysics Conference, Institute of BioMedical Sciences, Academia Sinica: Biophysical Society of R.O.C、IBMS, Academia Sinica, 2013-06-27 ~ 2013-06-29.
  24. Jen-Tse Huang*, 2013, “The different faces of the intrinsically disordered TDP-43 in Amyotrophic Lateral Sclerosis”, paper presented at Academia Sinica and UC Davis Neuroscience Symposium, University of california, San Francisco: UC Davis Neuroscience ,NPAS, 2013-04-22 ~ 2013-04-24.
  25. Yi-Ni Li , Chia-Sui Sun , Chu-Ya Wu , Joseph J.-T. Huang* , 2012, “The influence of molecular chaperones on the mHtt protein aggregation process”, paper presented at 2012 Annual Meeting of Chemical Society, Department of Chemistry, National Cheng-Kung University: Department of Chemistry, National Cheng-Kung University, 2012-12-01 ~ 2012-12-02.
  26. Jen-Tse Huang* , 2012, “Co-translational Protein Folding: The Journey from the Ribosome Tunnel to Cellular Function”, 71 pages, paper presented at The 12th Annual Meeting of the Protein Science Society of Japan, Nagoya, Japan: Protein Science Society of Japan, 2012-06-20 ~ 2012-06-23.
  27. Yi-Chen Huang, Jen-Tse Huang*, 2012, “Exploring the roles of DNA/RNA on TDP-43 aggregation in Amyotrophic Lateral Sclerosis”, 64 pages, paper presented at The 12th Annual Meeting of the Protein Science Society of Japan, Nagoya, Japan: Protein Science Society of Japan, 2012-06-20 ~ 2012-06-23.
  28. Liu, G. C.-H., Huang, J. J-T.*, 2012, “Characterization of the aggregation properties of amyloid core in the TDP-43 C-terminal fragments”, paper presented at The 17th Biophysics Conference, Institute of BioMedical Sciences, Academia Sinica: Biophysical Society of R.O.C、IBMS, Academia Sinica, 2012-05-23 ~ 2012-05-25.
  29. Joseph Jen-Tse Huang, 2012, “The impact of mutations in TDP-43 fragments on the formation of amyloid fibers and cellular toxicity”, paper presented at The 17th Biophysics Conference, Institute of BioMedical Sciences, Academia Sinica: Biophysical Society of R.O.C、IBMS, Academia Sinica, 2012-05-23 ~ 2012-05-25.
  30. Ku-Feng Lin, Joseph Jen-Tse Huang* , 2012, “Co-translational protein folding within the ribosome tunnel influences trigger-factor recruitment”, paper presented at The 17th Biophysics Conference, Institute of BioMedical Sciences, Academia Sinica: Biophysical Society of R.O.C、IBMS, Academia Sinica, 2012-05-23 ~ 2012-05-25.
  31. Joseph Jen-Tse Huang*, 2012, “Exploring the Co-translational Protein Folding Process with Novel Biophysical and Chemical Approaches”, paper presented at Frontiers in Chemistry-A symposium dedicated to the memory of Prof. Ta-shue Chou, 中央研究院化學所周大紓講堂: 中央研究院化學所, 2012-02-21.
  32. Huang, J.-T., 2011, “Applying Novel Biophysical and Chemical Approaches in Co-translational Protein Folding Studies”, paper presented at 2011Annual Meeting of Chemical Society, National Tsing Hua University, Hsinchu: National Tsing Hua University, 2011-12-03 ~ 2011-12-04.
  33. Huang, J.-T., 2011, “The Discovery of the Amyloid Fibrils from the C-terminal Fragments of TDP-43 in Amyotrophic Lateral Sclerosis”, paper presented at The 1st International Kyoto Conference on Organic Nanostructures, Kyoto University, Kyoto, Japan: Kyoto University, 2011-11-10.
  34. Huang, J.-T., 2011, “The Discovery of the Amyloid Fibrils from the C-terminal Fragments of TDP-43 in Amyotrophic Lateral Sclerosis”, paper presented at The 11th Tateshina Conference on Organic Chemistry, Nagoya, Japan: The 11th Tateshina Conference on Organic Chemistry, 2011-11-11 ~ 2011-11-13.
  35. Chia-Sui Sun, Ruei-Yu He, Yijuang Chern, Joseph J.-T. Huang*, 2011, “Characterize the aggregation process of the mHtt protein in the presence of chaperone”, paper presented at France-Taiwanesse Symposium on Neurobiology, IGBMC, France: IGBMC, 2011-11-28 ~ 2011-11-29.
  36. Huang, J.-T., 2011, “Light up the folding zone within the ribosome tunnel”, paper presented at Sunney Chan Symposium 2011, Chou Ta-Shue Lecture Hall, Institute of Chemistry, Academia Sini: Institute of Chemistry, Academia Sinica, 2011-10-05.
  37. Huang, J.-T., 2011, “The impact of co-translational protein folding within the ribosome tunnel on chaperone recruitment”, paper presented at The 16th Biophysics Conference, National Dong Hwa University, Hua Lien: Biophysical Society of Taiwan R.O.C, 2011-05-18 ~ 2011-05-21.
  38. Huang, J.-T., 2010, “ Explore the impact of ribosome tunnel on co-translational protein folding and chaperone recruitment”, paper presented at 2010 Annual meeting of Chinese Society, 台北: 台灣大學, 2010-12-04 ~ 2010-12-05.
  39. Huang, J.-T., 2010, “ Induction of amyloid fibrils by the TDP-43 fragments in ALS”, paper presented at 2010 Pacifichem Symposium, Honolulu, Hawaii, USA: Hawaii, USA, 2010-12-15 ~ 2010-12-20.
  40. Joseph Jen-Tse Huang, 2009, “The Induction of TDP-43 C-terminal Fragment into Amyloid Fibrils by G294A Mutation in Amyotrophic Lateral Sclerosis”, p27 pages, paper presented at Frontier of Protein Aggregation and Neurodegenerative Diseases, 中央研究院生物化學研究所: 中央研究院生物化學研究所、中央研究院生物醫學研究所、中央研究院細胞與個體生物學研究所、中央研究院化學研究所、中央研究院基因體中心、中央研究院分子生物研究所, 2009-06-04.
  41. Allan K.-H. Chen, Ryan Y.-Y. Lin, Eva Z.-J. Xie, and Joseph J.-T. Huang*, 2009, “Mutation in TDP-43 C-terminal Fragment can Induce Beta-Amyloid Fibril Formation”, paper presented at Joint International Conference of Biophysics and 14th Annual Conference of the Biophysical Society of ROC, 國立成功大學: Biophysical Society of ROC, 2009-06-24 ~ 2009-06-27.
  42. Ku-Feng Lin, Chia-Sui Sun, and Joseph Jen-Tse Huang* , 2009, “Study on Cotranslational Protein Folding within the Ribosomal Exit Tunnel”, paper presented at Joint International Conference of Biophysics and 14th Annual Conference of the Biophysical Society of ROC, 國立成功大學: Biophysical Society of ROC, 2009-06-24 ~ 2009-06-27.
技術報告
他類論文
  1. 黃人則、陳長謙、林谷峰, 2012, “蛋白質摺疊:從核醣體到細胞功能的旅程”, 中央研究院週報-知識天地專刊, 1364期.
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